Bacterial Pathogenesis: Methods and Protocols

ISBN-13: 9781588297402 / Angielski / Twarda / 2008 / 320 str.

Michael Otto

Opis

Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.

Recenzje

From the reviews:

"An eclectic collection of papers ranges from those with limited general applications, to those with a much broader interest. ... The additional notes in each paper are most useful, and may help those making use of these techniques avoid some of the frustrations associated with following methodologies in published journal papers. ... a volume to dip into seeking inspiration for new techniques to apply to one's own favourite organism." (Jonathan Fletcher, Microbiology Today, July, 2008)

"This book was written to bring scientists working in the field of bacterial pathogenesis up-to-date with both traditional tried-and-tested methods that have been in use for decades, and more recent technological advances and breakthroughs that have led to the development of new protocols. ... On the whole this book is very informative and includes an extensive set of complex and detailed protocols. ... I would recommend to anyone in the field or thinking of entering the field of bacterial pathogenesis." (Andrew Vaughan, Immunology News, August, 2008)

"The 431st volume of Methods in Molecular Biology published by Humana Press is dedicated to the problems of bacterial pathogenesis. ... Each chapter is concluded by an exhaustive list of references. ... By its high level of interest and schematic approach the book is suitable not only for scientists dealing with bacterial pathogenesis ... but it can be recommended also for graduate students in microbiology who are about to start their scientific career." (K. Tyllovâ, Folia Microbiologica, Vol. 53, November, 2008)

Spis treści

Part I. Mechanisms of Bacterial Pathogenesis 1 Isolation and Characterization of Lipopolysaccharides Michael A. Apicella 2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes Michelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee 3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus Susanne Engelmann and Michael Hecker 4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution Caitriona M. Guinane and J. Ross Fitzgerald 5 Detection and Inhibition of Bacterial Cell-Cell Communication Scott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg 6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsii James M. Battisti, Sandra J. Raffel, and Tom G. Schwan 7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferi Philip E. Stewart and Patricia A. Rosa 8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin (PIA) – A Molecular and Biochemical Approach Cuong Vuong and Michael Otto Part II. Host-Pathogen Interaction 9 Analysis of Staphylococcus aureus Gene Expression during PMN Phagocytosis Jovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo 10 Examining the Vector-Host-Pathogen Interface with Quantitative Molecular Tools Jason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch 11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages Jean Celli 12 Rate and Extent of Helicobacter pylori Phagocytosis Lee-Ann H. Allen 13 Culture, Isolation and Labeling of Anaplasma Phagocytophilum for Subsequent Infection ofHuman Neutrophils Dori L. Borjesson 14 Ultrastructural Analysis of Bacteria-Host Cell Interactions David W. Dorward 15 Infection of Human Monocyte-derived Macrophages with Coxiella burnetii Jeffrey G. Shannon and Robert A. Heinzen 16 Infection of Epithelial Cells with Salmonella enterica Olivia Steele-Mortimer 17 Determining the Cellular Targets of ROS in Borrelia burgdorferi Julie A. Boylan and Frank C. Gherardini Part III. Animal Models of Bacterial Infection 18 Bioluminescent Imaging of Bacterial Biofilm Infections in vivo Jagath L. Kadurugamuwa and Kevin P. Francis 19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans (Cotton Rat S. aureus Nasal Colonization Model) John Kokai-Kun 20 A Non-Human Primate Model of Acute Group A Streptococcus Pharyngitis Paul Sumby, Anne H. Tart, and James M. Musser Part IV. Identification of Therapeutic Targets and Typing Methods 21 Target-based Antimicrobial Drug Discovery Lefa E. Alksne and Paul M. Dunman 22 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa Typing Barun Mathema, Jose Mediavilla, and Barry N. Kreiswirth

Blurb

Bacterial infections affect world health today as a leading cause of morbidity and mortality. Pathogenic bacteria routinely command a broad spectrum of niches in the human host, making an understanding of pathogenesis mechanisms crucial to the development of prophylactics and treatment for bacterial diseases. A variety of in vitro methods, in vivo animal model systems and cutting-edge genomics assays have arisen in the effort to study bacterial pathogenesis and identify potential therapeutic targets. In Bacterial Pathogenesis, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections. Up-to-date molecular typing methods for Staphylococcus aureus and a new model of streptococcal pharyngitis in non-human primates are also included. Bacterial Pathogenesis will prove an invaluable collection for microbiologists, immunologists, cell biologists and infectious disease clinicians - and indispensable to all science researchers interested in studying pathogenic bacteria and related disease processes.