Bacterial Artificial Chromosomes: Volume 2: Functional Studies

ISBN-13: 9781617372896 / Angielski / Miękka / 2010 / 326 str.

Shaying Zhao; Marvin Stodolsky

Opis

Several developmental and historical threads are woven and displayed in these two volumes of Bacterial Artificial Chromosomes, the first on Library Construction, Physical Mapping, and Sequencing, and the second on Fu- tional Studies. The use of large-insert clone libraries is the unifying feature, with many diverse contributions. The editors have had quite distinct roles. Shaying Zhao has managed several BAC end-sequencing projects. Marvin Stodolsky during 1970 1980 contributed to the elucidation of the natural b- teriophage/prophage P1 vector system. Later, he became a member of the Genome Task Group of the Department of Energy (DOE), through which s- port flowed for most clone library resources of the Human Genome Program (HGP). Some important historical contributions are not represented in this volume. This preface in part serves to mention these contributions and also briefly surveys historical developments. Leon Rosner (deceased) contributed substantially in developing a PAC library for drosophila that utilized a PI virion-based encapsidation and tra- fection process. This library served prominently in the Drosophila Genome Project collaboration. PACs proved easy to purify so that they substantially replaced the YACs used earlier. Much of the early automation for massive clone picking and processing was developed at the collaborating Lawrence Berkeley National Laboratory. However, the P1 virion encapsidation system itself was too fastidious, and P1 virion-based methods did not gain popularity in other genome projects."

Spis treści

Use of BAC End Sequences for SNP Discovery Michael M. Weil, Rashmi Pershad, Ruoping Wang, and Sheng Zhao Exon Trapping for Positional Cloning and Fingerprinting Scott E. Wenderfer and John J. Monaco Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column Sally H. Cross BAC Microarray-Based Comparative Genomic Hybridization Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Daniel Pinkel, and Donna G. Albertson Large DNA Transformation in Plants Sangdun Choi Retrofitting BACs With a Selectable Marker for Transfection Zunde Wang, Angelika Longacre, and Peter Engler BAC Modification Using a RecA Expressing Shuttle Vector System Nathan Mise and Philip Avner Bacterial Artificial Chromosome Engineering Srividya Swaminathan and Shyam K. Sharan ET Recombination: DNA Engineering Using Homologous Recombination in E. coli Joep P. P. Muyrers, Youming Zhang, Vladmir Benes, Giuseppe Testa, Jeanette M. J. Rientjes, and A. Francis Stewart BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes Giuseppe Testa, Kristina Vintersten, Youming Zhang, Vladmir Benes, Joep P. P. Muyrers, and A. Francis Stewart Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes Kristina Vintersten, Giuseppe Testa, and A. Francis Stewart Generation of BAC Transgenic Mice Vikki M. Marshall, Janette Allison, Tanya Templeton, and Simon J. Foote BAC Rescue: A Tool for Functional Analysis of the Mouse Genome Deborah A. Swing and Shyam K. Sharan Herpesviruses: A Brief Overview Mathias Ackermann Cloning of b-Herpesvirus Genomes as Bacterial Artificial Chromosomes Eva-Maria Borst, Irena Crnkovic-Mertens, and Martin Messerle Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based AmpliconVectors Thomas G. H. Heister, Andrea Vögtlin, Lars Müller, Irma Heid, and Cornel Fraefel Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination) Markus Wagner and Ulrich H. Koszinowski Mutagenesis of Herpesvirus BACs by Allele Replacement Eva-Maria Borst, György Pósfai, Frank Pogoda, and Martin Messerle Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies Alistair McGregor and Mark R. Schleiss Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I Mark Suter and Hans Peter Hefti Index

Blurb

The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 2: Functional Studies provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 1: Library Construction, Physical Mapping, and Sequencing, presents readily reproducible techniques for library construction, physical mapping, and sequencing.
Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.