Bacterial Artificial Chromosomes

ISBN-13: 9781493954308 / Angielski / Miękka / 2016 / 348 str.

Kumaran Narayanan

Opis

Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls. Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.

Spis treści

Part I: BAC Construction and Characterization

 1     From Selective Full-Length Genes Isolation By TAR Cloning In Yeast To Their Expression From HAC Vectors In Human Cells

Natalay Kouprina, Nicholas C.O. Lee, Artem V. Kononenko, Alexander Samoshkin, and Vladimir Larionov

2     Recombineering Linear BACs

Qingwen Chen and Kumaran Narayanan

 

3     BAC Sequencing Using Pooled Methods

       Chris A. Saski, F. Alex Feltus, Laxmi Parida, and Niina Haiminen

 

Part II: Modification Of BACs

 

4     Making BAC Transgene Constructs With Lambda-Red Recombineering System

For Transgenic Animals Or Cell Lines

Scott Holmes, Suzanne Lyman, Jen-Kang Hsu, and Jrgang Cheng

 

5     Directing Enhancer-Traps And Itol2 End Sequences To Deleted BAC Ends With Loxp- and Lox511-Tn10 Transposons

  Pradeep K. Chatterjee

 

6     Recombining Overlapping BACs Into Single Large BACs

       George Kotzamanis and Athanassios Kotsinas

 

Part III: Gene Expression Using BACs as Vectors

 

7    Generation Of Knockout Alleles by RFLP Based BAC Targeting Of Polymorphic Embryonic Stem Cells

Tahsin Stefan Barakat and Joost Gribnau

 

8     Herpesvirus Mutagenesis Facilitated By Infectious Bacterial Artificial Chromosomes (Ibacs)

Karl E. Robinson and Timothy J. Mahony

 

9     Conversion Of BAC Clones Into Binary BAC (BIBAC) Vectors and Their Delivery Into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens

Shawkat Ali and Guus Bakkeren

 

10   Infectious Delivery of Alphaherpesvirus Bacterial Artificial Chromosomes

Kurt Tobler and Cornel Fraefel

 

Part IV: Applications Of BACs In Model Organisms, Medical Genetics, and Drug Discovery

11   A Recombineering-Based Gene Tagging System For Arabidopsis

Jose M. Alonso and Anna N. Stepanova

 

12   BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies

 Petra Kraus, Cecilia L Winata, and Thomas Lufkin

 

13   Bacs-On-Beads^TM (Bobs^TM) Assay for the Genetic Evaluation of Prenatal Samples And Products of Conception

Francesca Romana Grati, François Vialard, and Susan Gross

 

14   New BAC Probe Set To Narrow Down Chromosomal Breakpoints In Small and Large Derivative Chromosomes, Especially Suited for Mosaic Conditions

Ahmed B. Hamid, Xiaobo Fan, Nadezda Kosyakova, Gopakumar Radhakrishnan, Thomas Liehr, and Tatyana Karamysheva

 

15   BAC-Probes Applied For Characterization Of Fragile Sites (FS)

Kristin Mrasek, Kathleen Wilhelm, Luciana G. Quintana, Luise Theuss, Thomas Liehr, Andreja Leskovac, Jelena Filipovic, Gordana Joksic, Ivana Joksic, and Anja Weise

 

16   Application Of BAC-Probes To Visualize Copy Number Variants (CNVs)

Anja Weise, Moneeb A.K. Othman, Samarth Bhatt, Sharon Löhmer, and Thomas Liehr

 

17   Site-Specific Integration Of Bacterial Artificial Chromosomes Into Human Cells Bradley Mccoll, Sara Howden, and Jim Vadolas

 

18   Generation Of BAC Reporter Cell Lines For Drug Discovery

Betty R. Kao, Bradley Mccoll, and Jim Vadolas