Axon Degeneration: Methods and Protocols

ISBN-13: 9781071605844 / Angielski / Twarda / 2020 / 340 str.

Elisabetta Babetto

Spis treści

Part I: Introduction

 

1. Axon Degeneration: Which Method to Choose?

            Michael P. Coleman

 

Part II: In Vitro Assays

 

2. Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures

            Andrea Loreto and Jonathan Gilley

 

3. Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration

            Jonathan Gilley and Andrea Loreto

 

4. Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro

            Jung Eun Shin and Yongcheol Cho

 

5. Viral Transduction of DRG Neurons

            Yo Sasaki

 

6. Planning and Analysis of Axon Degeneration Screening Experiments

            Lyndah Lovell, Jack Bramley, and William Buchser

 

7. A Microfluidic Culture Platform to Assess Axon Degeneration

            Yu Yong, Christopher Hughes, and Christopher Deppmann

 

8. A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration

            Elisabetta Babetto

 

9. Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination

            Alex Clark

 

Part III: Ex Vivo Assays

 

10. Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage

            Claire S. Durrant

 

11. Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function

            Kosala N. Dissanayake, Robert Chang-Chih Chou, Rosalind Brown, and Richard R. Ribchester

 

12. Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction

            Vera Valakh and Johanna G. Flyer-Adams

 

13. Ex Vivo Studies of Optic Nerve Axon Electrophysiology

            Chinthasagar Bastian, Sylvain Brunet, and Selva Baltan

 

14. Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations

            Rodrigo López-Leal and Felipe A. Court

 

15. Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis

            Bogdan Beirowski

 

Part IV: In Vivo Assays

 

16. Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury

            Jon P. Niemi, Jane A. Lindborg, and Richard E. Zigmond

 

17. Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues

            Ying Cao and Jing Yang

 

18. Transmission Electron Microscopy and Morphometry of the CNS White Matter

            Julia M. Edgar, Rebecca Sherrard Smith, and Ian D. Duncan

 

19. In Vivo Calcium Imaging During Axon Degeneration in Zebrafish

            Mauricio E. Vargas

 

20. In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves

            James N. Sleigh, Andrew P. Tosolini, and Giampietro Schiavo

 

21. In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons

            Rupkatha Banerjee, Joseph A. White II, and Shermali Gunawardena

 

22. A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster

            Martha R.C. Bhattacharya

 

23. Models of Axon Degeneration in Drosophila Larvae

            E.J. Brace and Aaron DiAntonio

 

24. In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster

            Mary A. Logan and Sean D. Speese

Blurb

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration.

Chapters 2, 3, and 20 are available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.