Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols

ISBN-13: 9781493993826 / Angielski / Miękka / 2019 / 228 str.

Kursad Turksen

Opis

This detailed book compiles state-of-the-art protocols from researchers actively working in the area of autophagy, a crucial cellular process that regulates numerous cellular functions. In order to accelerate advances in the field, the volume explores aspects of autophagy research where a better understanding of its role is vitally important, such as in the maintenance of stem cell subpopulations and the regulation of differentiation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Autophagy in Differentiation and Tissue Maintenance: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of this key cell biological action.

Spis treści

1. Visualization and Measurement of Multiple Components of the Autophagy Flux

            Tracey Evans, Robert Button, Oleg Anichtchik, and Shouqing Luo

 

2. Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells

            Kiran Suhas Nilangekar and Bhupendra V. Shravage

 

3. Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells

            Rohan S. Kulkarni, Manmohan S. Bajaj, and Vaijayanti P. Kale

 

4. Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement

            Kristyn Gumpper, Matthew Sermersheim, Michael X. Zhu, and Pei-Hui Lin

 

5. Autophagy in Adipocyte Differentiation

            Zhipeng Tao, Longhua Liu, Louise D. Zheng, and Zhiyong Cheng

 

6. Assessing Autophagy in the Leydig Cells

            Hui Gao, Chao Liu, and Wei Li

 

7. Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

            Sinem Tunçer and Sreeparna Banerjee

 

8. Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells

            Javad Alizadeh, Shahla Shojaei, Adel Sepanjnia, Mohammad Hashemi, Eftekhar Eftekharpour, and Saeid Ghavami

 

9. Autophagy in Zebrafish Extraocular Muscle Regeneration

            Alfonso Saera-Vila, Phillip E. Kish, and Alon Kahana

 

10. The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry

            Melek Ozturk, Ozlem Dagdeviren Ozsoylemez, and Fatma Kaya Dagistanli

 

11. Cloning of Autophagy-Related MicroRNAs

            Deniz Gulfem Ozturk, Muhammed Kocak, and Devrim Gozuacik

 

12. Assays to Monitor Aggrephagy in Drosophila Brain

            Anne-Claire Jacomin and Ioannis P. Nezis

 

13. Methods for Monitoring Autophagy in Silkworm Organs

            Aurora Montali, Morena Casartelli, Daniele Bruno, Annalisa Grimaldi, and Gianluca Tettamanti

 

14. Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia

            Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin, Yongquan Hu, Tian Meng, Baojin Wu, Rongrong He, and Du Feng

 

15. Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening

            Georgios Konstantinidis, Sonja Sievers, and Yao-Wen Wu

 

16. Porcine Cell-Free System to Study Mammalian Sperm Mitophagy

            Won-Hee Song and Peter Sutovsky

 

17. Monitoring and Measuring Mammalian Autophagy

            Sujit K. Bhutia, Prakash P. Praharaj, Chandra S. Bhol, Debasna P. Panigrahi, Kewal K. Mahapatra, Srimanta Patra, Sarbari Saha, Durgesh N. Das, Subhadip Mukhopadhyay, Niharika Sinha, Prashanta K. Panda, and Prajna P. Naik