Automation of Cytogenetics

ISBN-13: 9783642747403 / Angielski / Miękka / 2012 / 316 str.

Claes Lundsteen; Jim Piper

Opis

This book complements the successful series of Workshops on the Au tomation of Cytogenetics which have been sponsored for more than a decade by the European Community (EC). The contributed papers all arise from pre sentations at one of the last three such Workshops, in Berlin (1986), Cortona (1987) and Langollen (1988). Collaborative cytogenetics automation research and development activities in Europe are now supported by the "Concerted Action in Automation of Cytogenetics" (CAACG), as one of the activities of the EC's COMAC-BME committee which supervises the coordination of re search in biomedical engineering within the Medical Technology Development target (project no. 11.1.1/13). The structure of EC programme for medical and health research is described on the following pages. The book presents the state of the art in the automation of cytogenet ics, comprising 20 papers by researchers, developers and users of cytogenetics automation. Topics covered include evaluations of complete image-based chro mosome analysis systems which have resulted from the successful collaboration between European researchers and industry, current active research into new image analysis techniques (with particular emphasis on techniques for auto mated segmentation of chromosome clusters), the development of systems for chromosome aberration scoring, automatic specimen preparation, and the use of flow cytometry for chromosome measurement and analysis."

Spis treści

I Automatic Chromosome Aberration Scoring.- Automated aberration scoring : the requirements of an end-user.- An Automated Chromosome Aberration Scoring System.- Automatic High Resolution Digitisation of Metaphase Cells for Aberration Scoring and Karyotyping.- II Karyotyping Systems: Progress and Evaluation.- Athena: A Macintosh-Based Interactive Karyotyping System.- Recent Advances in Chromosome Analysis with the Chromoscan.- Proposal for Evaluation of Cytogenetic Laboratories in Order to Plan the Installation and Determine the Cost Effectiveness of Automated Chromosome Analysis Systems.- A Cost Evaluation of Magiscan 2.- III Flow Cytogenetics.- The Flow Cytometry Approach to Automated Chromosome Analysis.- Flow Analysis of Human Chromosomes.- Chromosome Aberration Detection with Hybridized DNA Probes: Digital Image Analysis and Slit Scan Flow Cytometry.- IV Automatic Preparation of Cytogenetic Specimens.- An Automated System for the Culturing and Harvesting of Human Chromosome Specimens.- Evaluation and Development of a System for Automated Preparation of Blood Specimens for Cytogenetic Analysis.- V Automatic Chromosome Segmentation.- Decomposition of Overlapping Chromosomes.- Resolution of Composites in Interactive Karyotyping.- Separation of Touching Chromosomes.- Model-Based Contour Analysis in a Chromosome Segmentation System.- VI Chromosome Classification.- On the use of Automatically Inferred Markov Networks for Chromosome Analysis.- Density Profiles in Human Chromosome Analysis.- Cytogenetic Analysis by Automatic Multiple Cell Karyotyping.- Towards a Knowledge-Based Chromosome Analysis System.- Bibliography - Cytogenetics Automation 1975–88.