Autoimmunity: Methods and Protocols

ISBN-13: 9781607617198 / Angielski / Twarda / 2012 / 504 str.

Andras Perl

Opis

Continued refinement of wide-spread access to transgenic technology has allowed for new animal models have been developed that exhibit features of autoimmune disease have been developed that exhibit features of autoimmune disease. The second edition of Autoimmunity: Methods and Protocols researchers in the field detail many of the most up-to-date methods which are now commonly used to study autoimmunity. The first half the book focuses on methods and protocols used to assess immunological and biochemical pathways of diseases pathogenesis in human subjects. While the second half investigates treatment of inflammatory arthritis, experimental allergic encephalomyelitis (EAE), IDDM, scleroderma, and uveitis in animal models and assessment of genetic, immunological, and biochemical parameters underlying spontaneous or exogenous antigen-induced diseases. Written in the highly successful Methods in Molecular Biology(tm) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Through and intuitive, Autoimmunity: Methods and Protocols, Second Edition seeks to aid scientists in the autoimmunity field to extract new meaning of old models and developing new ones.

Spis treści

1. Pathogenesis and Spectrum of Autoimmunity

Andras Perl

2. Mapping Susceptibility Gene in Systemic Lupus Erythematosus

R. Hal Scofield and Kenneth M. Kaufman

3. Methods and Protocols to study T Cell Signaling Abnormalities in Human Systemic Lupus Erythematosus

Vaishali R. Moulton, Mindy S. Lo, and George C. Tsokos

4. Assessment of Mitochondrial Dysfunction in Lymphocytes of Patients with Systemic Lupus Erythematosus

Andras Perl, Robert Hanczko, and Edward Doherty

5. The Role of Endocytic Recycling in Autoimmunity

Tiffany Telarico, Andras Perl

6. Multi-Parameter Flow Cytometry and Bioanalytics for B Cell Profiling in Systemic Lupus Erythematosus

Denise A. Kaminski, Chungwen Wei, Alexander Rosenberg, F. Eun-Hung Lee, and Ignacio Sanz

7. Experimental Use of Mouse Models of Systemic Lupus Erythermatosus

Standford L. Peng

8. Murine Models of Lupus Induced By Hypomethlated T Cell

(DNA hypomethylation and lupus....)

Bruce Richardson, Amr H Sawalha, Donna Ray and Raymond Yung

9. Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity

Czeslawa Kowal and Betty Diamond

10. Analysis of Renal Mononuclear Phagocytes in Murine Models of SLE

Ramalingam Bethunaickan, Ranjit Sahu and Anne Davidson

11. A Murine Autoimmune Model of Rheumatoid Arthritis and Systemic Lupus Erythematosus Associated with Deregulated Production of IL-17 and IL-21

Partha S. Biswas, Kyuho Kang, Sanjay Gupta, Govind Bhagat, and Alessandra B. Pernis

 ^{12. The Parent-into-F1 Murine Model in the Study of Lupus-like Autoimmunity and CD8 Cytotoxic T Lymphocyte Function}

^{Kateryna Soloviova, Maksym Puliaiev, Anthony Foster, Charles S. Via}

 ^{13. Genetic Approach to Study Lupus Glomerulonephritis Yan Ge, Michael G. Brown, Hongyang Wang,  PhD and Shu Man Fu}

14. Animal Models of Primary Biliary Cirrhosis:  Materials and Methods

Patrick S.C. Leung, Guo Xiang Yang, Amy Dhirapong, Koichi Tsuneyama, William  M. Ridgway, and M. Eric Gershwin

 15. Modeling Innate Immunity in Murine Skin: Utilization of Subcutaneous Osmotic Pumps for Inflammatory and Fibrotic Skin Disease

Michael Dimarzio, Giuseppina Farina, Robert Lafyatis

 16. Flow Cytometric Identification of Fibrocytes in Scleroderma Lung Disease

Thomas M. Russell, Erica L. Herzog, Richard Bucala

 17. Oxidative Stress and Beta Cell Dysfunction

Yaíma L. Lightfoot, Jing Chen, Clayton E. Mathews

18. Experimental Autoimmune Encephalomyelitis

Praveen Rao, Ph.D and Benjamin M. Segal

 19. Mouse Models of Multiple Sclerosis:  Experimental Autoimmune Encephalomyelitis and Theiler’s Virus-Induced Demyelinating Disease Derrick P. McCarthy, Maureen H. Richards, and Stephen D. Miller

20. Pathorgenesis of Multiple Sclerosis What Can we Learn from the Cuprizone Model

Peter Acs, Bernadette Kalman

 21. Assessing Inflammatory Disease at Mucosal Surfaces in Murine Genetic Models

Engelman, R.W., and Kerr, W.G.

 22. Rodent Models of Experimental Autoimmune Uveitis (EAU)

Rajeev K. Agarwal, Phyllis B. Silver and Rachel R. Caspi

 23. Tolerance Induction via B-cell Delivered Gene Therapy

Robert J. Rossi, Belinda M. Jackson, Ai-Hong Zhang, and David W. Scott