Auditory and Vestibular Research: Methods and Protocols

ISBN-13: 9781493936137 / Angielski / Twarda / 2016 / 547 str.

Bernd Sokolowski

Opis

This second edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methods and Protocols, Second Edition guides readers through protocols on cell culture, tissue engineering, nanotechnology, high-throughput screening, and physiology. Chapters on physiology cover techniques that include optical coherence tomography, patch clamping, and photostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Auditory and Vestibular Research: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Spis treści

 Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates.- Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.-  A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear.-  Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma.- RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays .- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System.-  Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research.- Protein Quantification of the Developing Cochlea by Mass Spectrometry.-  Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy.- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins.- The Single-Molecule Approach to Membrane Protein Stoichiometry.- Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy.- Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue.- Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments.-  Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy.-  Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate.- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates.- The Use of Human Wharton’s Jelly Cells for Cochlear Tissue Engineering.- Nanotechnology in Auditory Research: Membrane Electromechanics in Hearing.- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders.-  Development of Cell-based High-Throughput Chemical Screens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.- Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo.- Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos.-  Whole Cell Patch Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti.-  Glass Probe Stimulation of Hair Cell Stereocilia.- A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells.- In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs).- Mapping Auditory Circuits with Photostimulation of Caged Glutamate.