Atlas of Confocal Laser Scanning In-Vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophtalmology

ISBN-13: 9783540327059 / Angielski / Twarda / 2006 / 216 str.

Rudolf F. Guthoff; Joachim Stave; Christophe Baudouin

Opis

Confocal microscopy with laser scanning technology yields in-vivo images of ocular and ocular adnexal surfaces that are so brilliant that they rival histology in terms of quality.This unique atlas and textbook demonstrates normal in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies various cellular structures using cell-density calculations and establishes correlations between novel optical sections of various diseases of the ocular surface and clinical findings. Furthermore, it supports the interpretation of novel high-magnification optical sections by comparing corneal and conjunctival imprint cytology with in-vivo images and describes early inflammatory changes in corneal grafts, as well as corneal conjunctivalisation in limbal stem cell deficiency, corneal dystrophies or infections, flap interface and margin characteristics after laser in-situ keratomileusis (LASIK). In addition, it instructs the reader about diagnostic and therapeutic follow-up strategies and provides a brief introduction to applications in other fields such as dentistry and ear, nose and throat surgery.

Spis treści

Introduction. -. Principles of confocal in-vivo microscopy: Slit scanning techniques. - Laser-scanning imaging plus pachymetry. - Basics of image formation in confocal in-vivo microscopy. - Non-contact Confocal Laser Scanning Microscopy. - Konfokale Fluoreszenzmikroskopie. . - General anatomical considerations. - Ex-vivo applications: Blood components. - Pthogens. - Ocular structures. - In-vivo confocal laser-scanning microscopy: Tearfilm (Normal anatomy, Pathological findings) Epithelium (Normal anatomy. - Pathological findings). - Corneal nerves (Normal anatomy, Subepithelial, Stromal, Pathological findings). - Bowman’s Membrane (Normal anatomy, Pathological findings). - Stroma (Normal anatomy, Pathological findings) Descemet Membrane (Normal anatomy, Pathological findings, Endothelium and trabecular structures, Normal anatomy,Pathological findings). - Intraocular structures ( Lens, Iris) (Normal anatomy, Pathological findings, Limbal Region, Normal anatomy, Pathological findings). - Conjunctiva (Normal anatomy, Pathological findings) Lid (Normal anatomy, Pathological findings). - Promising Clinical Applications: Glaucoma surgery. - . - Alterations of the cornea on contact lens wearers Applications outside Ophthalmology: Skin. - Oral Mucosa and Tongue. - Gingiva and Teeth. - In-vivo Confocal Microscopy in animal experiments: Rabbits. - Rats. - Mice. – Monkeys. - Current and Future Developments: Three-dimensional confocal laser scanning microscopy. - Functional imaging. - Outlook