Arabidopsis Protocols

ISBN-13: 9781071608821 / Angielski / Miękka / 2021

Sanchez-Serrano, Jose J.

Opis

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

Spis treści

Part I: Arabidopsis Resources

 

1. Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses

Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, and David Somers

 

2. Bioinformatic Tools in Arabidopsis Research

G. Alex Mason, Alex Cantó-Pastor, Siobhan M.  Brady, and Nicholas J. Provart

 

Part II: Genetic Techniques in Arabidopsis

 

3. Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene

Carlos Alonso-Blanco, Belén Méndez-Vigo, and F. Xavier Picó

 

4. Non-sterile Grafting Methods for Arabidopsis  

Nien-Chen Huang, and Tien-Shin Yu

 

5. CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana

Daisuke Miki, Gaurav Zinta, Fangnan Peng, Zhengyan Feng, and Jian-Kang Zhu

 

6. Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration

César Bernat-Silvestre, Vanessa Vieira, Fernando Aniento, and Mª Jesús Marcote

 

7. Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts

Yonglun Zeng, Changyang Ji, Youshun Lin, and Liwen Jiang

 

8. Photosynthetic and Heterotrophic Cell Suspension Cultures

Enrico Cortese, Luca Carraretto, Barbara Baldan, and Lorella Navazio

 

9. Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals

Tak Lee and Insuk Lee

 

Part III: Molecular and  Cell biological Techniques in Arabidopsis

 

10. Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana

Anis Meschichi and Stefanie Rosa

 

11. Identification and Quantification of Small RNAs

Di Sun, Zeyang Ma, Jiaying Zhu, and Xiuren Zhang

 

12. Flow Cytometry and Sorting in Arabidopsis

David W. Galbraith and Guiling Sun

 

13. Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development

Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, and Marcus G. Heisler

 

14. Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging

Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt, Wolf B. Frommer, and Masayoshi Nakamura

 

15. Using Tripartite Split-sfGFP for the Study of Membrane Protein–Protein Interactions

Tzu-Yin Liu

 

16. High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling

Marisa S. Otegui

 

17. Atomic Force Microscopy to Study Cell Wall Mechanics in Plants

Mateusz Majda

 

18. Identification and Characterization of Reproductive Mutations in Arabidopsis

María Flores‑Tornero, Stefanie Sprunck, and Thomas Dresselhaus

 

Part IV: Biochemical and Physiological Techniques in Arabidopsis

 

19. Metabolomic Analysis of Natural Variation in Arabidopsis

Si Wu, Saleh Alseekh, Yariv Brotman, and Alisdair R. Fernie

 

20. Untargeted Metabolomics of Arabidopsis Stomatal Immunity

Lisa David, Jianing Kang, and Sixue Chen

 

21. Biotin-based Proximity Labeling Of Protein Complexes in Planta

Madiha Khan, Rajagopal Subramaniam, and Darrell Desveaux

 

22. Phosphoproteomic Analysis of Plant Membranes

Lin Xi, Waltraud X. Schulze, and Xu Na Wu

 

23. RNA-binding Protein Immunoprecipitation and High Throughput Sequencing

Tino Köster and Dorothee Staiger