Apoptosis and Cancer: Methods and Protocols

ISBN-13: 9781493916603 / Angielski / Twarda / 2014 / 208 str.

Gil Mor; Ayesha Alvero

Opis

In Apoptosis and Cancer: Methods and Protocols, Second Edition, expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only in vitro but also in vivo, optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Apoptosis and Cancer: Methods and Protocols, Second Edition aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research.

Spis treści

1. Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-induced Apoptosis

 Gaurav S. Choudhary, Sayer Al-harbi, and Alexandru Almasan

 

2. Flow Cytometry Enumeration Of Apoptotic Cancer Cells By Apoptotic Rate

David Diaz, Alfredo Prieto, Eduardo Reyes, Hugo Barcenilla, Jorge Monserrat,  and Melchor Alvarez-Mon

 

3. “Multiplexed Viability, Cytotoxicity, and Caspase Activity Assays”

Andrew L. Niles and Terry L. Riss

 4. A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation using Live Content Imaging

Katherine Artymovich and Daniel M. Appledorn

5. Detection of End Stage Apoptosis by ApopTag^® TUNEL Technique

Chandra Mohan, Kevin Long, Manpreet Mutneja, and Jun Ma

 6. Detection and Quantification of Apoptosis in Primary Cells using Taqman® Protein Assay

Christina Pfister, Heike Pfrommer, Marcos S. Tatagiba, and Florian Roser

 

7. Detection of p53 Protein Aggregation in Cancer Cell Lines and Tumor Samples

Yang Yang-Hartwich, Jamie Bingham, Francesca Garofalo, Ayesha B. Alvero, and Gil Mor

 

8. Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation

Yang Yang-Hartwich, Emily Romanoff, Jamie Bingham, Ayesha B. Alvero, and Gil Mor

 

9. Homogeneous, Bioluminescent Proteasome Assays

Martha A. O’Brien, Richard A. Moravec, Terry L. Riss, and Robert F. Bulleit

 

10. Laser Capture Microdissection for Gene Expression Analysis

Mallikarjun Bidarimath, Andrew K. Edwards,and Chandrakant Tayade

 

11. Using the Peggy Simple Western System for Fine Needle Aspirate Analysis

Erik T. Gentalen and John M. Proctor

 

12. Analysis of Autophagosome Formation Using Lentiviral Biosensors for Live Fluorescent Cellular Imaging

Kevin Long, Chandra Mohan, Janet Anderl, Karyn Huryn-Selvar, Haizhen Liu, Kevin Su, Mark Santos, Matthew Hsu, Lucas Armstrong, and Jun Ma

 

13. Optical Imaging of Ovarian Cancer Using HER-2 Affibody Conjugated Nanoparticles

Minati Satpathy, Rafal Zielinski,  Ilya Lyakhov, and Lily Yang

 

14. Measuring Cardiac Autophagic Flux in vitro and in vivo

Michael A. Gurney, Chengqun Huang, Jennifer M. Ramil, Nandini Ravindran, Allen M. Andres, Jon Sin, Phyllis-Jean Linton, and Roberta A. Gottlieb

15. PET Imaging for Tyrosine Kinase Inhibitor (TKI) Biodistribution in Mice

Hiroshi Fushiki, Yoshihiro Murakami, Sosuke Miyoshi, and Shintaro Nishimura