Apoptosis Techniques and Protocols

ISBN-13: 9781588290120 / Angielski / Twarda / 2002 / 250 str.

Andrea C. LeBlanc; Andra C. LeBlanc

Opis

In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.

Recenzje

Review of the first edition:
"The book is useful for an actively working neuroscientist who is going to study apotosis and has enough experience to choose the most suitable models and protocols among all the diverse offerings of the book." Three Stars. -Doody's Health Sciences Book Review Journal

"...the book has updated many of the state-of-the-art techniques for analysis of neuronal apoptosis. More importantly, the book also offers the first-hand and step-by-step experimental details for molecular, cellular, biochemical and immunohistochemical assays of neuronal apoptotic death." - Biotechnic & Histochemistry

Spis treści

APOPTOSIS TECHNIQUES AND PROTOCOLS, 2ND EDITION Edited by: Andréa C. LeBlanc CONTENTS Preface to the Series Preface Contributors Molecular Characterization of the Proapoptotic Protein Bax Yi-Te Hsu and Soraya Smaili Assessing Cytochrome-c Release from Mitochondria Douglas W. Ethell and Douglas R. Green Monitoring Caspases in Neuronal Cell Death Younes Bounhar, Omar Tounekti, and Andréa C. LeBlanc HSV Amplicon Vectors in Neuronal Apoptosis Studies Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons Yan Zhang and Andréa C. LeBlanc Designing Microarray Experiments for Neurobiology P. Scott Eastman and Jeanne F. Loring Analysis of Gene Expression by Nylon Membrane cDNA Arrays H. Michael Tucker and Steven Estus Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs Mark D. Berry and Paula C. Ashe Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons Barbara Karten, Bronwyn L. MacInnis, Hubert Eng, Yoko Azumaya, Grace Martin, Karen Lund, Russell C. Watts, Jean E. Vance, Dennis E. Vance, and Robert B. Campenot Detection and Analysis of Synaptosis Greg M. Cole and Karen Gylys In Situ Detection of Apoptotic Neurons Kevin A. Roth Apoptotic and Oxidative Indicators in Alzheimer's Disease Arun K. Raina, Lawrence M. Sayre, Craig S. Atwood, Catherine A. Rottkamp, Ayala Hochman, Xiongwei Zhu, Mark E. Obrenovich, Shun Shimohama, Akihiko Nunomura, Atsushi Takeda, George Perry, and Mark A. Smith Index

Blurb

The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature.
Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.