Apoptosis: Methods and Protocols, Second Edition

ISBN-13: 9781617794896 / Angielski / Miękka / 2011 / 400 str.

P. Erhard;Ambrus Toth

Opis

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death."

Recenzje

From the reviews of the second edition:

"Provides step-by-step protocols for identifying apoptotic cells and analyzing their role in normal tissue development and disease. ... The book is written primarily for basic science and clinical researchers (graduate students, postdoctoral fellows, scientists) ... . Biomedical researchers working in radiation biology, pharmacology, and molecular pathology also will value this book because of its focus on apoptosis in various organs including the heart and brain. ... This beautiful up-to-date synthesis of information about the analysis of apoptosis is filled with practical information." (Bruce A. Fenderson, Doody's Review Service, July, 2010)

Spis treści

I. Detection of Apoptosis 1. Analyzing Caspase Activation and Caspase Activity in Apoptotic Cells Sharad Kumar and Loretta Dorstyn 2. Flow Cytometry-Based Apoptosis Detection Donald Wlodkowicz, Joanna Skommer, and Zbigniew Darzynkievicz 3. Live to Dead Cell Imaging Stephen W.G. Tait, Lisa Bouchier-Hayes, Andrew Oberst, Samuel Connell, and Douglas R. Green 4. Detection of Apoptosis in Tissue Sections Eva Csizmadia and Vilmos Csizmadia 5. Detection of Apoptosis in Cell-Free Systems Dhyan Chandra and Dean G. Tang II. Detection of Non-Apoptotic Cell Death 6. Methods to Analyze Cellular Necroptosis Benchun Miao and Alexei Degterev 7. Detection of Cell Death by Autophagy Narasimman Gurusamy and Dipak K. Das III. Modifications of Apoptotic Proteins during Apoptosis 8. Methods to Analyze Transglutamination of Proteins Involved in Apoptosis Zoltan Nemes and Laszlo Fesus 9. Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis Neelam Azad, Anand Krishnan V. Iyer, and Yon Rojanasakul 10. Application of in vivo EPR for Tissue pO2 and Redox Measurements Nadeem Khan and Dipak K. Das IV. Analysis of the Function of Major Regulators of Apoptosis/Cell Survival 11. Assays to Measure p53-dependent and Independent Apoptosis Darren . Phillips, Sean P. Garrison, John R. Jeffers, and Gerard P. Zambetti 12. Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis Ramon Roset and Gabriel Gil-Gomez 13. Fluorimetric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis Soumya SinhaRoy and Gyorgy Hajnoczky 14. Regulation of Apoptosis by the Unfolded Protein Response Andrew Fribley, Kezhong Zhang, and Randal Kaufman 15. Detection of Uncoupling Protein-2 (UCP-2) as a Mitochondrial Modulator of Apoptosis Zoltan Derdak, Tamako A. Garcia and Gyorgy Baffy 16. A Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis Riccardo Spizzo and George Calin 17. Assessment of Apoptotic Cell Phagocytosis by Macrophages Kathleen A. McPhilips and Lars-Peter Erwig V. Analysis of Apoptosis in Different Organs 18. Detection of Apoptosis in Mammalian Development Lin Lin, Carlos Penalosa, Yixia Ye, Richard A. Lockshin, and Zahra Zakeri 19. Detection of Apoptosis in the Central Nervous System Youngsoo Lee and Peter J. McKinnon 20. Genetic Mapping of Anti-apoptosis Pathways in Myeloid Progenitor Cells Dan Liu and Zhou Songyang 21. Analysis of Apoptosis in Isolated Primary Cardiac Myocytes Adel Mandl, Ambrus Toth, and Peter Erhardt 22. Cell Death in Myoblasts and Muscles Lawrence M. Schwartz, Zhengliang Gao, Christine Brown, Sangram S. Parelkar, and Honor Glenn VI. Analysis of Apoptosis in Model Organisms 23. Reliable Method for Detection of Programmed Cell Death in Yeasts Xinchen Teng and J. Marie Hardwick 24. Detection of Cell Death in Drosophila Kimberly McCall, Jeanne S. Peterson, and Tracy L. Pritchett 25. Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans Nan Lu, Xiaomeng Yu, Xiangwei He, and Zheng Zhou 26. Detection of Herpes Simplex Virus Dependent Apoptosis Christopher R. Cotter and John A. Blaho