Antiviral Resistance in Plants: Methods and Protocols

ISBN-13: 9781493996377 / Angielski / Miękka / 2020 / 292 str.

Kappei Kobayashi;Masamichi Nishiguchi

Opis

This detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel mechanisms of plant virus resistance, methods for the analysis and practical use of RNA silencing, as well as methods for the development of plant viral vaccines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond.

Spis treści

Section 1.        Analysis of natural virus resistance mechanisms

1          Identification and functional analysis of NB-LRR-type virus resistance genes

                        Overview and Functional analysis of candidate genes

2-4       Analysis of resistance signaling pathways and resistance mechanisms

            2          Genetic analysis in Arabidopsis thaliana

              3            VIGS analysis in Nicotiana benthamiana      

            4          Resistance mechanisms

5          Identification and functional analysis of recessive virus resistance genes

                        Overview and analytical methods

6-7       Identification of non-conventional virus resistance genes

            6          Screening in diverse plants

            7          Biochemical identification of virus resistance genes

8-9       Isolation and analysis of virus resistance breaking strains

            8          Analysis of field isolates and Error-prone PCR-based mutagenesis

            9          Forced evolution in plants and selection in grafted scions

10-12   Analysis of RNA silencing-mediated virus resistance

            10        Analysis of small RNAs (small RNA gel blotting, Deep sequencing)

            11        Analysis of DNA methylation and histone modification in virus resistance

            12        Functional analysis of RNA silencing machinery

 

Section 2.        Engineered virus resistance and Virus vaccine: Design and Evaluation

13        RNA silencing-based virus resistance with viral RNA and DNA targets

14        Virus resistance by CRISPR/Cas9 targeting viral sequences

15        Resistance breeding through the control of host factors involved in virus replication

16        Virus resistance breeding through CRISPR/Cas9-mediated genome editing

17        Attenuated viruses: Isolation, evaluation and mechanisms

18        RNA silencing-mediated virus vaccine