Antigen Processing and Presentation Protocols

ISBN-13: 9780896037458 / Angielski / Twarda / 2000 / 270 str.

Joyce C. Solheim

Opis

Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules). To investigate such a diverse array, it is necessary that biochemical, cell biology, and immunological techniques all be employed. The purpose of Antigen Processing and Presentation Protocols is therefore to detail the most up-to-date techniques being used in this burgeoning field. Such techniques include those used to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to MHC/peptide. Antigen Processing and Presentation Protocols should aid both those new and those experienced in this area of research in extending the questions that can be asked and answered by the application of these current methods. For editorial assistance, I would like to thank Angela Beninga and Rachael Turnquist.

Spis treści

Purification of 20S Proteasomes, Jill R. Beyette, Timothy Hubbell, and John J. Monaco. Use of Proteasome Inhibitors to Examine Processing of Antigens for Major Histocompatibility Complex Class I Presentation, Luis C. Antón, Jack R. Bennink, and Jonathan W. Yewdell. Tracing the Route Taken by Peptides and Major Histocompatibility Complex Class I Molecules in the Presentation of Exogenous Antigens, Christopher C. Norbury. Functional Analysis of Antigen Processing and Major Histocompatibility Complex Class II-Restricted Presentation, John D. Lich and Janice S. Blum. Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA, Diane N. Turvy and Janice S. Blum. In Vitro Generation of Functional Human and Murine Dendritic Cells, Seema S. Ahuja. Genetic Engineering of Dendritic Cells Using Retrovirus-Based Gene Transfer Techniques, Seema S. Ahuja. Use of Vaccinia Virus Expression Vectors to Investigate Antigen Processing and Presentation, E. John Wherry, Deepa Rajagopal, and Laurence C. Eisenlohr. Sindbis Virus-Based Vectors for the Study of Class I Antigen Presentation In Vitro and In Vivo, John M. Polo and Cornelia C. Bergmann. Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci via Direct DNA Sequencing, Mary Ellexson-Turner, David Sidebottom, Sean Turner, Tara Bennett, and William H. Hildebrand. Photolabeling the Transporter Associated with Antigen Processing, Vashti G. Lacaille and Matthew J. Androlewicz. Application of One-Dimensional Isoelectric Focusing to Separate Different Major Histocompatibility Complex Class I Alleles and Determine Their Allelic Interactions with Transporter Associated with Antigen Processing (TAP), Anne Neisig and Jacques Neefjes. Analysis of Major Histocompatibility Complex Class I Interactions with Endoplasmic Reticulum Proteins, Heth R. Turnquist and Joyce C. Solheim. Methods of Purification of Heat Shock Protein-Peptide Complexes for Use as Vaccines Against Cancers and Infectious Diseases,Pramod K. Srivastava and Navdeep S. Jaikaria. Random Peptide Libraries: A Tool for Analyzing the Peptide Specificity of Major Histocompatibility Complex Class I Molecules , James Stevens and Geoffrey W. Butcher. Neural Network Method for Predicting Peptides that Bind Major Histocompatibility Complex Molecules, Kamalakar Gulukota and Charles DeLisi. Analysis of T-Cell Response Using Altered Peptide Ligands, Florence Boisgérault, Natalie Anosova, Eugenia V. Fedoseyeva, Robert C. Tam, and Gilles Benichou. Assays of Thymic Selection: Fetal Thymus Organ Culture and an In Vitro Thymocyte Dulling Assay, Kristin A. Hogquist. Protocol for Diversion of a CD4+ Response to the T-Helper 2 Cell Pathway, Francesca Galbiati and Luciano Adorini. Quantitating Presentation of MHC Class I-Restricted Antigens, Weisan Chen, Jack R. Bennink, and Jonathan W. Yewdell. Identification of CD8+ T-Cell-Stimulating Antigen Genes in cDNA Libraries, Lisa M. Mendoza, Subramaniam Malarkannan, and Nilabh Shastri. Generation of Antigen-Specific, LacZ-Inducible T-Cell Hybrids, Subramaniam Malarkannan, Lisa M. Mendoza, and Nilabh Shastri. Index.

Blurb

The processing and presentation of antigens by major histocompatibility complex (MHC) molecules is a crucial immunological phenomenon in infectious disease, malignancy, autoimmune disease, and transplantation. In Antigen Processing and Presentation Protocols, well-recognized and innovative experimentalists detail their cutting-edge methods for studying this complex process. Drawing on expertise from biochemistry, cell biology, and immunology, they describe step-by-step methods designed to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T cell response to the MHC/peptide complex. Each method is presented in sufficient detail to be readily reproducible and includes notes about potential pitfalls and tips on how to avoid failures. Emphasis is given those technical steps critical for experimental success that are often omitted from methods published in the primary literature.
Eminently accessible and state-of-the-art, Antigen Processing and Presentation Protocols provides both new and experienced investigators with highly practical tools that will extend the questions that can be asked, and effectively be answered, concerning antigen processing/presentation.