Antigen Processing: Methods and Protocols

ISBN-13: 9781493994526 / Angielski / Miękka / 2020 / 458 str.

Peter van Endert

Opis

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
Stefan Tenzer and Hansjörg Schild

2. Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry
Kathrin Textoris-Taube, Ulrike Kuckelkorn, Christin Keller, and Peter-M. Kloetzel 

3. Trimming of MHC Class I Ligands by ERAP Aminopeptidases
Mirjana Weimershaus, Irini Evnouchidou, Peter van Endert, and Marlene Bouvier

4. Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases
Mirco Compagnone and Doriana Fruci

5. Testing the Impact of Protease Inhibitors in Antigen Presentation Assays
Michael Basler and Marcus Groettrup

6. In Vitro Studies of MHC Class I Peptide Loading and Exchange 
Marlene Bouvier

7. Measuring Synthesis and Degradation of MHC Class I Molecules
Simon J. Powis and Antony N. Antoniou 

8. Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules
Takayuki Kanaseki and Nilabh Shastri

9. A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics
Devin Dersh, Jonathan W. Yewdell, and Jiajie Wei

10. Purification and Identification of Naturally Presented MHC Class I and II Ligands
Annika Nelde, Daniel J. Kowalewski, and Stefan Stevanović

11. Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs
Michael Ghosh, Moreno Di Marco, and Stefan Stevanović

12. Quantitating MHC Class I Ligand Production and Presentation using TCR-Like Antibodies
Brian P. Dolan

13. Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors
Nathalie Vigneron, Vincent Stroobant, Wenbin Ma, Alexandre Michaux, and Benoît J. Van den Eynde

14. A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway 
Patrique Praest, Hendrik de Buhr, and Emmanuel J.H.J. Wiertz

15. Evaluating CD8+ T Cell Responses In Vitro
François A. Lemonnier

16. Recombinant Poxviruses: Versatile Tools for Immunological Assays
Katherine S. Forsyth, Mary M. Addison, and Laurence C. Eisenlohr

17. Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
Maria Embgenbroich, Jasmin Mertins, Thorsten Lang, and Sven Burgdorf

18. Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases
Evelina Gatti

19. Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays
Kyung-Jin Cho and Paul A. Roche

20. Monitoring Protein Endocytosis and Recycling using FACS-Based Assays
Even Walseng and Paul A. Roche

21. In Vitro Digestion with Proteases Producing MHC Class II Ligands
Mira Tohmé, Sophia Maschalidi, and Bénédicte Manoury

22. Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors
Sebastian Temme, Nadine Temme, and Norbert Koch

23. Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
Tieying Hou, Cornelia Rinderknecht, Debopam Ghosh, Andreas V Hadjinicolaou, Robert Busch, and Elizabeth D. Mellins

24. Studying MHC Class II Peptide Loading and Editing In Vitro
AeRyon Kim, Isabel Ishizuka, Isamu Hartman, Yuri Poluektov, Kedar Narayan, and Scheherazade Sadegh-Nasseri

25. Monitoring Antigen Processing for MHC Presentation via Macroautophagy
Monique Gannage, Rosa Barreira da Silva, and Christian Münz

26. Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies 
Ying Ying Kong and William W. Kwok

27. Production of Antigen Specific Human CD4+ T Cell Lines and Clones
Jessica Matthis, Victoria King, and Helena Reijonen

28. Monitoring Dendritic Cell Activation and Maturation
Christophe Macri, Ben Fancke, Kristen J. Radford, and Meredith O’Keeffe

29. Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes
Juan José Sáez, Ana-Maria Lennon-Duménil, and Maria-Isabel Yuseff

30. Analyzing Antigen Recognition by Natural Killer T Cells
Sebastian Zeissig and Richard S. Blumberg