Antibody Phage Display: Methods and Protocols

ISBN-13: 9781617794933 / Angielski / Miękka / 2011 / 240 str.

Robert Aitken

Opis

In Antibody Phage Display expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display.

Recenzje

From the reviews of the second edition:

"This book presents a wide range of methods of isolating recombinant antibodies from phage display libraries, examining how the targets of these antibodies can be identified, and how they can be used. ... This is clearly a book meant for individuals in research laboratories. ... provide a resource for innovative investigators to help them use this methodology to answer their questions. ... As always, the very detailed experimental methods and the notes section with its caveats are the high points of each chapter." (Marion C. Cohen, Doody's Review Service, December, 2009)

"This new volume delineates more the practical applications of the technique ... . targets a very specific audience - those who use monoclonal antibodies and are looking for a technique that can harness the power of unique epitope specificity within an easily malleable IgG domain module, together with the utility of ex vivo screening/selection from variable domain libraries ... . provides a superb collection of techniques, all annotated with the extensive practical hints based on personal experience that we have come to expect in this series." (Jonathan S. Duke-Cohan, Immunology News, May, 2010)

Spis treści

Table of Contents Preface Contributors Antibody phage display – overview of a powerful technology that has quickly translated to the clinic Beatrix Kotlan and Mark C. Glassy Design and construction of synthetic phage-displayed Fab libraries Jenny Bostrom and Germaine Fuh Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies Henrik J. Ditzel Construction of phage antibody repertoires from the blood of West Nile virus infected donors Mark Throsby and John de Kruif Anti-ß2GP-I and anti-prothrombin antibodies generated by phage display Javier Cabiedes and Antonio R. Cabral The generation and selection of single-domain, V region libraries from nurse sharks Martin F. Flajnik and Helen Dooley The isolation of scFvs against small target molecules Iain Shaw and Marian Kane Display and selection of scFv antibodies on HEK-293T cells Mitchell Ho and Ira Pastan Isolation of scFvs that inhibit the NS3 protease of Hepatitis C virus by a combination of phage display and a bacterial genetic screen Limor Nahary, Alla Trahtenherts and Itai Benhar Guided selection methods through chain shuffling Bao Guo-qiang and He Xian-Li Sequential antigen panning for selection of broadly cross-reactive HIV-1 neutralizing human monoclonal antibodies Mei-Yun Zhang and Dimiter S. Dimitrov Selection of antibodies able to rapidly enter mammalian cells Marie-Alix Poul Identification of the specificity of isolated phage display single chain antibodies using yeast 2-hybrid screens Nicolaj Rasmussen and Henrik J.Ditzel Generation of bispecific and tandem diabodies Sergey M. Kipriyanov Intrabody expression in eukaryotic cells Laurence Guglielmi and Pierre Martineau High level periplasmic expression and purification of scFvs Sergey M. Kipriyanov Expression of single-chain Fv fragments in E. coli cytoplasm Laurence Guglielmi and Pierre Martineau High-level expression of a phage display-derived scFv in Pichia pastoris Leonardo M. Damasceno, Frank Lee, Gerd Ritter, Lloyd Old, and Carl Batt

O autorze

Robert Aitken, einer der bedeutendsten Zen-Meister der Gegenwart, lebt auf Hawaii und praktiziert seit über 60 Jahren Zen. Mehrere Veröffentlungen auf Deutsch erschienen.

Blurb

Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of  immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition, expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and execution of experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can enter cells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Detailed and innovative, Antibody Phage Display: Methods and Protocols, Second Edition is a critical handbook on phage display technology which is certain to stimulate the reader’s imagination as much as it will guide future practice in the laboratory.