Antibody Methods and Protocols

ISBN-13: 9781493959303 / Angielski / Miękka / 2016 / 325 str.

Gabriele Proetzel; Hilmar Ebersbach

Opis

This Methods in Molecular Biology (TM) volume covers in vitro and in vivo generation of antibodies, as well as techniques for screening, analysis and modification of antibodies and antibody fragments. Offers materials lists, protocols and troubleshooting tips.

Spis treści

1. Antigen Presentation for the Generation of Binding Molecules

            Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang

 

2. Recombinant Antibodies and In Vitro Selection Technologies

            C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R.M. Bradbury, and Sachdev S. Sidhu

 

3. Phage Display

            Konstantin Petropoulos

 

4. Generation of Human Fab Libraries for Phage Display

            Christoph Rader

 

5. Selection of Human Fab Libraries by Phage Display

            Christoph Rader

 

6. Ribosome Display

            George Thom and Maria Groves

 

7. Hybridoma Technology for the Generation of Monoclonal Antibodies

            Chonghui Zhang

 

8. The Application of Transgenic Mice for Therapeutic Antibody Discovery

            E-Chiang Lee and Michael Owen

 

9. Production of Human or Humanized Antibodies in Mice

            Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné

 

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

            Elisabetta Traggiai

 

11. Kinetic Screening in the Antibody Development Process

            Michael Schräml and Matthias Biehl

 

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

            Michael Schräml and Leopold von Proff

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Konstantin Petropoulos

 

4. Generation of Human Fab Libraries for Phage Display

            Christoph Rader

 

5. Selection of Human Fab Libraries by Phage Display

            Christoph Rader

 

6. Ribosome Display

            George Thom and Maria Groves

 

7. Hybridoma Technology for the Generation of Monoclonal Antibodies

            Chonghui Zhang

 

8. The Application of Transgenic Mice for Therapeutic Antibody Discovery

            E-Chiang Lee and Michael Owen

 

9. Production of Human or Humanized Antibodies in Mice

            Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné

 

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

            Elisabetta Traggiai

 

11. Kinetic Screening in the Antibody Development Process

            Michael Schräml and Matthias Biehl

 

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

            Michael Schräml and Leopold von Proff

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogné

 

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

            Elisabetta Traggiai

 

11. Kinetic Screening in the Antibody Development Process

            Michael Schräml and Matthias Biehl

 

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

            Michael Schräml and Leopold von Proff

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Elisabetta Traggiai

 

11. Kinetic Screening in the Antibody Development Process

            Michael Schräml and Matthias Biehl

 

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

            Michael Schräml and Leopold von Proff

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Michael Schräml and Matthias Biehl

 

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

            Michael Schräml and Leopold von Proff

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Michael Schräml and Leopold von Proff

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

 

13. Determination of Antibody Glycosylation by Mass Spectrometry

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, and Hans Koll

 

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Jiahui Yang and Christoph Rader

 

15. PEGylation of Antibody Fragments for Half-Life Extension

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Simona Jevševar, Mateja Kusterle, and Maja Kenig

 

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

 

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

 

18. Methods to Engineer and Identify IgG₁ Variants with Improved FcRn Binding or Effector Function

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Robert F. Kelley and Y. Gloria Meng

 

19. Class-Specific Effector Functions of Therapeutic Antibodies

            Virginie Pascal, Brice Laffleur, and Michel Cogné

            Virginie Pascal, Brice Laffleur, and Michel Cogné

Blurb

The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.