Antibody Arrays: Methods and Protocols

ISBN-13: 9781071610664 / Angielski / Miękka / 2021

Whittaker, Kelly C.

Opis

This detailed book presents a technical overview and practical methodology of a variety of antibody array formats and technologies. As advantages and disadvantages of antibody array types are explored, the volume also delves into practical applications of antibody arrays pertaining to investigations of specific research topics and biological processes as well as guidance on the methods of processing, analysis, and storage of array data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Antibody Arrays: Methods and Protocols aims to empower the reader with the information required to select the most appropriate array for their research application, with the technical knowledge to use and process the array, and with the knowledge to perform analysis that realizes the maximum benefit from the data generated.

Spis treści

1. Sandwich-Based Antibody Arrays for Protein Detection

            Ying Qing Mao, Brianne Petritis, and Ruo-Pan Huang

 

2. Well-Based Antibody Arrays

            Robert S. Matson

 

3. One-Step Antibody Arrays

            Ying Qing Mao and Lin-hai Li

 

4. Global Detection of Proteins by Label-Based Antibody Array

            Zhizhou Kuang, Li-Pai Chen, Ruochun Huang, and Ruo-Pan Huang

 

5. Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen

            Anastasiia Halushkina, William Buchmann, Nathalie Jarroux, and Régis Daniel

 

6. Multiplexed Protein Biomarker Detection with Microfluidic Electrochemical Immunoarrays

            Abby Jones, Patricia Czarnecki, Lasangi Dhanapala, and James F. Rusling

 

7. Cytometry Multiplex Bead Antibody Array

            Benyue Zhang, Gang Xiao, Ying Qing Mao, Zhiqiang Lv, and Ruo-Pan Huang

 

8. Antibody Arrays: Barcode Technology

            Liwei Yang and Jun Wang

 

9. Reverse Phase Protein Arrays

            Justin B. Davis, Sydney Andes, and Virginia Espina

 

10. Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression

            Ruochun Huang, Chunhui Gao, Wenjuan Wu, and Ruo-Pan Huang

 

11. Methods for Membranes and Other Absorbent Surfaces

            Jarad J. Wilson, Yun Xi, Ruochun Huang, and Ruo-Pan Huang

 

12. Precise Chip-to-Chip Reagent Transfer for Cross-Reactivity-Free Multiplex Sandwich Immunoassays

            François Paquet-Mercier, David Juncker, and Sébastien Bergeron

 

13. Antibody Printing Technologies

            Valentin Romanov and Benjamin D. Brooks

 

14. Self-Assembling Peptide Hydrogels for 3D Microarrays

            Greta Bergamaschi, Alessandro Strada, Roberto Frigerio, Marina Cretich, and Alessandro Gori

 

15. Surface Modification of Glass Slides with Aminosilanes for Microarray Use

            Chris Stuart

 

16. Dried Blood-Based Protein Profiling Using Antibody Arrays

            Kelly C. Whittaker, Ying Qing Mao, Siwei Zhu, Zhiqiang Lv, and Ruo-Pan Huang

 

17. Profiling Glycoproteins on Functionalized Reverse Phase Protein Array

            Ying Zhang, Liyuan Zhang, Anton Iliuk, and W. Andy Tao

 

18. Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin

            Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike

 

19. Analyzing Signaling Pathways Using Antibody Arrays

            Hao Tang, Chaohui Duan, Zhizhou Kuang, and Ruo-Pan Huang

 

20. Using Antibody Arrays for Biomarker Discovery

            Shuhong Luo, Ling Yunbiao, Li-Pai Chen, and Ruo-Pan Huang

 

21. Immune Cell Isolation from Murine Intestine for Antibody Array Analysis

            Joshua A. Owens and Rheinallt M. Jones

 

22. Database Development for Antibody Arrays

            Zhaowei Xu, Likun Huang, and Sheng-ce Tao

 

23. Data Analysis for Antibody Arrays

            Huihua Zhang, Ying Qing Mao, Brianne Petritis, and Ruo-Pan Huang

 

24. Statistical Analysis Options for Antibody Array Data

            Jingqiao Lu