Antibiotics: Methods and Protocols

ISBN-13: 9781071628546 / Angielski / Twarda / 2022 / 405 str.

Peter Sass

Opis

This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols,  Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.

Spis treści

Part I: Production and Design

1. Antibiotics - precious goods in changing times

Peter Sass

 

2. A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycetes Culture Supernatants

Anika Rütten, Wolfgang Wohlleben, Lena Mitousis, and Ewa Maria Musiol-Kroll

 

3. Sampling of Human Microbiomes to Screen for Antibiotic-Producing Commensals

Benjamin-Torres Salazar, Anna Lange, Laura Camus, and Simon Heilbronner

 

4. Production of Antimicrobial Compounds by Homologous and Heterologous Expression

I Dewa M. Kresna, Zerlina G. Wuisan, and Till F. Schäberle

 

5. Isolation and Purification of Natural Products from Microbial Cultures

Thomas Schafhauser and Andreas Kulik

 

6. Structure Elucidation of Antibiotics

Julia Moschny, Georgios Daletos, Peter Proksch, and Chambers C. Hughes

 

7. Computer-Aided Drug Design – An update

Wenbo Yu,David J. Weber, and Alexander D. MacKerell, Jr.

 

8. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents

Alexander Zipperer, Jasmin Scheurer, and Dorothee Kretschmer

 

Part II: Mode of Action and Resistance

9. Microscopy-based Multiwell Assay to Characterize Disturbed Bacterial Morphogenesis Upon Antibiotic Action

Cruz L. Matos de Opitz and Peter Sass

 

10. Expansion Microscopy of Bacillus subtilis

Viola Middelhauve, Jan-Peter Siebrasse and Ulrich Kubitscheck

 

11. Tracking global and local changes in membrane fluidity through fluorescence spectroscopy and microscopy

Madeleine Humphrey, Ireny Abdelmesseh Nekhala, Kathi Scheinpflug, Oxana Krylova, Ann-Britt Schäfer, Jessica A. Buttress, Michaela Wenzel, and Henrik Strahl

 

12. Quantitative analysis of microscopy data to evaluate bacterial responses to antibiotic treatment

Dominik Brajtenbach, Jan-Samuel Puls, Cruz L. Matos de Opitz, Peter Sass, Ulrich Kubitscheck, and Fabian Grein

 

13. Application of a Bacillus subtilis whole-cell biosensor (P_liaI-lux) for the identification of cell wall active antibacterial compounds

Carolin Martina Kobras, Sali May Morris, Thorsten Mascher, and Susanne Gebhard

 

14. Determination of Bacterial Membrane Impairment by Antimicrobial Agents

Miriam Fuerst-Wilmes and Hans-Georg Sahl

 

15. A colorimetric assay to identify and characterize bacterial primase inhibitors

Allan H. Pang and Oleg V. Tsodikov

 

16. Cell-based fluorescent screen amenable to HTS to Identify Inhibitors of Bacterial Translation Initiation

Matteo Raneri, Emilio Alvarez-Ruiz, Danuta Mossakovska, and Federica Briani

 

17. ­Bacterial two component systems: Overexpression, purification − in vitro and in vivo inhibitor screens

Alina Dietrich, Mike Gajdiss, Michael Türck, Ian Monk, and Gabriele Bierbaum

 

18. Sample preparation for mass-spectrometry based absolute quantification of bacterial proteins in antibiotic stress research

Sandra Maaß, Minia Antelo Varela, Florian Bonn, and Dörte Becher

 

19. Elemental analysis for the characterization of antimicrobial effects

Christoph H. R. Senges and Julia E. Bandow

 

20. label-free quantitation of ribosomal proteins from bacillus subtilis for antibiotic research

Sina Schäkermann, Pascal Dietze, and Julia E. Bandow

 

21. Functional Metagenomics to Study Antibiotic Resistance

Bejan Mahmud, Manish Boolchandani, Sanket Patel, and Gautam Dantas