Antibiotic Resistance Protocols

ISBN-13: 9781493960729 / Angielski / Miękka / 2016 / 227 str.

Stephen H. Gillespie; Timothy D. McHugh

Opis

This book fully updates and builds upon its first edition. Beginning with chapters on epidemiology and population genetics, it continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance.

Recenzje

From the reviews:

"...an excellent, well-produced book, full of protocols on a range of modern molecular techniques, applicable not only to research on antibiotics resistance, but to general microbiology....The articles are of a uniformly high standard, well illustrated and referenced, which act as logical easy to follow protocols. this book is a must for anyone involved in research on antibiotic resistance; equally all microbiologists who use modern molecular methods should consult it in the expectation of finding well-described established techniques and numerous cutting-edge developments." - MicroBiology Today

From the reviews of the second edition:

"This is a well produced book ... . The articles are of a uniformly high level, well illustrated and referenced, working with logical and easy to follow protocols. ... This book is excellent for anyone involved in research on antibiotic resistance, whether students, researchers or professionals in the field of microbiology who use modern molecular techniques. Readers can find answers to queries on a number of well-defined and established cutting-edge techniques." (Elsa Masae Mamizuka, Brazilian Journal of Pharmaceutical Sciences, Vol. 46 (4), October-December, 2010)

"This is the second edition of a protocol book that details the methods used to detect antibiotic resistance mechanisms in bacteria. ... This book is written for scientists working in the area of antibiotic development and bacterial resistance mechanisms. ... a sound book with good advice for investigators working in the field of antimicrobial development. It provides a good basis for the study of bacterial survival mechanisms and how bacteria adapt to different environments." (Rebecca T. Horvat, Doody's Book Reviews, August, 2010)

Spis treści

Part I: Epidemiology and Population Genetics 1. The RM Test for Determining Methicillin-Resistant Staphylococcus aureus (MRSA) Lineages Jodi A. Lindsay and Julia M.-L. Sung 2. Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx Bambos M. Charalambous and Ndeky M. Oriyo Part II: Genomics and Gene Expression 3. Methods to Determine Antibiotic Resistance Gene Silencing Virve I. Enne and Peter M. Bennett 4. Studying Gene Induction of Glycopeptide Resistance Using Gene Swapping Hee-Jeon Hong 5. Real Time PCR Methods to Study Expression of Genes Related to Hypermutability Denise O’Sullivan 6. Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial Compounds Simon. J. Waddell and Philip D. Butcher Part III: Fitness Mutation and Physiology 7. Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity Mirjana Macvanin and Diarmaid Hughes 8. Measurements of Heme Levels and Respiration Rate in Salmonella enterica serovar Typhimurium Diarmaid Hughes and Mirjana Macvanin 9. Methods to Determine Fitness in Bacteria Cassie F. Pope, Timothy D. McHugh, and Stephen H. Gillespie 10. Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosis Joanna Bacon, Kim A. Hatch, and Jon Allnutt Part IV: Detection of Resistance 11. Identification of Efflux-Mediated Multi-Drug Resistance in Bacterial Clinical Isolates by Two Simple Methods Marta Martins, Isabel Couto, Miguel Viveiros, and Leonard Amaral 12. Evaluation of Efflux Activity of Bacteria by a Semi-Automated Fluorometric System Miguel Viveiros, LilianaRodrigues, Marta Martins, Isabel Couto, Gabriella Spengler, Ana Martins, and Leonard Amaral 13. Measuring the Activity of Active Efflux in Gram-Negative Bacteria Mark A. Webber and Nick G. Coldham 14. Rapid Characterization of b -Lactamases by Multiplex PCR Neil Woodford 15. Rapid Methods for Testing Inhibitors of Mycobacterial Growth Dimitrios Evangelopoulos and Sanjib Bhakta 16. Use of Biofilm Model Systems to Study Antimicrobial Susceptibility Jonathan Pratten and Derren Ready 17. Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing® Catherine Arnold

Blurb

Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.