Antibacterial Peptide Protocols

ISBN-13: 9781617370274 / Angielski / Miękka / 2010 / 259 str.

William M. Shafer

Opis

As this book. Antibacterial Peptide Protocols, will attest, my enthusi asm for the field of antibacterial peptides is based on a conviction (and I am unashamed to say, prejudice) that these substances are in essence antibiotics produced by the host that then participate in host defense against infectious agents. Because of their capacity to exert antibiotic-like action against patho genic microorganisms (bacteria, fungi, parasites, and viruses), there is reason to believe that these agents will soon be used clinically to treat infectious diseases. In fact, in recent years, biotechnology companies have been formed for the sole purpose of developing antibacterial peptides for clinical use. It should be emphasized that antibacterial peptides will likely play a major role in the treatment of infectious diseases, particularly with the increasing prob lem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies. The topic of this volume of Methods in Molecular Biology, the diverse methods used in research on antibacterial peptides, is thus quite timely. As the subject of antibacterial peptides develops into its own discipline (something strongly suggested by the explosion in the number of papers published over the past decade), it is essential that reliable techniques and strategies be made available not only to those of us in the field, but also to the newcomers and researchers in complementary disciplines."

Spis treści

Origins and Development of Peptide Antibiotic Research: From Extracts to Abstracts to Contracts, John K. Spitznagel. Part I. Isolation and Characterization of Antibacterial Peptides. HPLC Methods for Purification of Antimicrobial Peptides, Michael E. Selsted. Strategies for the Isolation and Characterization of Antimicrobial Peptides of Invertebrates, Charles Hetru and Philippe Bulet. Big Defensin and Tachylectins-1 and -2, Shun-ichiro Kawabata and Sadaaki Iwanaga. Tachyplesin and Anti-Lipopolysaccharide Factor, Tatsushi Muta and Sadaaki Iwanaga. Circular Dichroism Studies of Secondary Structure of Peptides, Martha M. Juban, Maryam M. Javadpour, and Mary D. Barkley. Analytical Ultracentrifugation Studies of Association of Peptides, Martha M. Juban, Maryam M. Javadpour, and Mary D. Barkley. NMR Characterization of Amphipathic Helical Peptides, Xiaotang Wang and Kathleen M. Morden. Part II. Molecular Biology of Antibacterial Peptides. Laboratory Production of Antimicrobial Peptides in Native Conformation, Erika V. Valore and Tomas Ganz. An Approach Combining Rapid cDNA Amplification and Chemical Synthesis for the Identification of Novel, Cathelicidin-Derived, Antimicrobial Peptides, Alessandro Tossi, Marco Scocchi, Margherita Zanetti, Renato Gennaro, Paola Storici, and Domenico Romeo. Molecular Biological Strategies in the Analysis of Antibiotic Peptide Gene Families: The Use of Oligonucleotides as Hybridization Probes, Charles L. Bevins and Gill Diamond. Part III. Assay Systems for Studying Antibacterial Peptides. Designer Assays for Antimicrobial Peptides: Disputing the 'One-Size-Fits-All' Theory, Deborah A. Steinberg and Robert I. Lehrer. Interaction of Cationic Peptides with Bacterial Membranes, Shafique Fidai, Susan W.Farmer, and Robert E. W. Hancock. The Genetic Basis of Microbial Resistance to Antimicrobial Peptides, Eduardo A. Groisman and Arden Aspedon. Assay of Antibacterial Activities of the Bactericidal/Permeability-Increasing Proeting in Natural Biological Fluids, Jerrold Weiss. Assay Systems for Measurement of Chemotactic Activity, H. Anne Pereira. Neutralization of the In Vivo Activity of E. coli-Derived Lipopolysaccharide by Cationic Peptides, Daniel J. Brackett, Megan R. Lerner, and H. Anne Pereira. Index.

Blurb

In Antibacterial Peptide Protocols, leading authorities review for the first time in one volume all the major biochemical, molecular, bacteriological, and physical techniques available to assess antimicrobial peptides. These state-of-the-art methods ensure easily reproducible results in such important procedures as the isolation and characterization of antimicrobial peptides, the molecular characterization of genes encoding antimicrobial peptides, and the use of expression systems to isolate peptides. Bioassays and microbial genetic techniques are also included, as are antibacterial assays as the final readout system.

These methods detailed in Antibacterial Peptide Protocols will play an important role in the treatment of infectious diseases, particularly with the increasing problem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies.