Anti-Biofilm Potential of Sesbania grandiflora Against S.aureus : Assessment of Sesbania grandiflora Aqueous Extract Induced Modification in Extracellular Polymeric Substance of S.aureus

ISBN-13: 9786138331803 / Angielski / Miękka / 2018 / 92 str.

Kubendiran Lavanya; Srinivasan Venkatesan; Babujanarthanam Ranganathan

Opis

The main objective of this research is to investigate the anti-biofilm and anti-bacterial activity of Sesbania grandiflora (S. grandiflora) against Staphylococcus aureus. S. grandiflora extract was prepared and analyzed with UV -Vis spectroscopy, Fourier transform infrared spectroscopy, Dynamic light scattering. Biofilm forming pathogens were identified by congo-red assay. Quantification of Extracellular polymeric substance (EPS) particularly protein and carbohydrate were calculated. The efficacy of the herbal extract S. grandiflora and its inhibition against the pathogenic strain of S. aureus was also evaluated. The gradual decrease or disappearance of peaks reveals the reduction of protein and carbohydrate content in the EPS of S. aureus when treated with S. grandiflora. The antibacterial activity of S. grandiflora extract against the bacterial strain S. aureus showed that the extract was more active against the strain. To conclude, anti-biofilm and antibacterial efficacy of S. grandiflora plays a vital role in biofilm producing pathogens and act as a good source for controlling the microbial population.

O autorze

Ms.K. Lavanya is a full-time Ph.D. research scholar at Department of Environmental science, Periyar University, Salem, Tamil Nadu, India. She has obtained her M.Sc degree in Thiruvalluvar University, Vellore, Tamilnadu, India. Her area of research is Extraction and Production of Extracellular Polymeric Substances and its Prospective Applications.