Animal Models of Diabetes: Methods and Protocols

ISBN-13: 9781071603840 / Angielski / Twarda / 2020 / 342 str.

Aileen King

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Practical Considerations when Using Mouse Models of Diabetes

Aileen J.F. King, Lydia F. Daniels Gatward, and Matilda R. Kennard

2. An Overview of Rodent Models of Obesity and Type 2 Diabetes

Thomas A. Lutz

3. Selectively Bred Diabetes Models: GK Rats, NSY Mice, and ON Mice

Mototsugu Nagao, Jonathan Lou S. Esguerra, Anna Wendt, Akira Asai, Hitoshi Sugihara, Shinichi Oikawa, and Lena Eliasson

4. A Review of Mouse Models of Monogenic Diabetes and ER Stress Signalling

Paraskevi Salpea, Cristina Cosentino, and Mariana Igoillo-Esteve

5. Rat Models of Human Type 1 Diabetes 

Sigurd Lenzen, Tanja Arndt, Matthias Elsner, Dirk Wedekind, and Anne Jörns

6. Mouse Models of Autoimmune Diabetes: The Non-Obese Diabetic (NOD) Mouse 

Dawei Chen, Terri C. Thayer, Li Wen, and F. Susan Wong

7. Mouse Models of Virus-Induced Type 1 Diabetes

Gustaf Christoffersson and Malin Flodström-Tullberg

8. Rat Models of Virus-Induced Type 1 Diabetes

James C. Needell and Danny Zipris

9. Large Animal Models of Diabetes

Barbara Ludwig, Eckhard Wolf, Uwe Schönmann, and Stefan Ludwig

10. Use of Streptozotocin in Rodent Models of Islet Transplantation 

Aileen King, Elisabet Estil-les, and Eduard Montanya

11. Transplantation of Islets of Langerhans into the Anterior Chamber of the Eye for Longitudinal In Vivo Imaging 

Christian M. Cohrs, Chunguang Chen, and Stephan Speier

12. Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish

Sumeet Pal Singh and Nikolay Ninoy

13. Generating Beta-Cell Specific Transgenic Mice Using the Cre-Lox System

Lorna I.F. Smith, Thomas G. Hill, and James E. Bowe

14. The Glucose Tolerance Test in Mice

Patricia Fonseca Pedro, Anastasia Tsakmaki, and Gavin A. Bewick

15. Assessment of Insulin Tolerance In Vivo in Mice

Irene Cózar-Castellano and Germán Perdomo

16. Continuous Glucose Monitoring in Conscious Unrestrained Mice

Aileen J.F. King, Matilda R. Kennard, and Manasi Nandi

17. Assessing Mouse Islet Function

Patricio Atanes, Inma Ruz-Maldonado, Oladapo E. Olaniru, and Shanta J. Persaud

18. Assessing Immune Responses in the Nonobese Diabetic Mouse Model of Type 1 Diabetes

Terri C. Thayer, Dimitri Kakabadse, Joanne Boldison, and F. Susan Wong

19. Assessment of Insulin Tolerance Ex Vivo

Irene Cózar-Castellano and Germán Perdomo

20. Using Pancreas Tissue Slices for the Study of Islet Physiology

Julia K. Panzer, Christian M. Cohrs, and Stephan Speier

21. Determining Beta Cell Mass, Apoptosis, Proliferation, and Individual Beta Cell Size in Pancreatic Sections

Noèlia Téllez and Eduard Montanya

Subject Index List…

Blurb

This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models. The chapters in this book cover topics such as important considerations when working with mouse models of diabetes, highlighting factors that new investigators may not be aware of and some potential pitfalls in experimental outcomes; main characteristics of some commonly used animal models of diabetes research, ranging from mice to primates; animal models used to study specific aspects of beta-cell biology; and a focus on techniques used to assess blood glucose homeostasis, insulin action, and islet function in vivo and ex vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Animal Models of Diabetes: Methods and Protocols is a valuable resource that will help diabetes researchers design and carry out in vivo studies that will best suit their experimental questions and needs.