Animal Cell Culture Techniques

ISBN-13: 9783540630081 / Angielski / Miękka / 1998 / 618 str.

Martin Clynes

Opis

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.

Spis treści

Animal Cell Culture Techniques (Springer Lab Manual), Clynes Ed. 1 Cloning Animal Cells, Shirley McBride, Mary Heenan, Martin Clynes. 2 Cell Culture and Diagnostic Virology, Edwin OKelly. 3 Screening for Mycoplasma Contamination in Animal Cell Cultures, Mary Heenan and Martin Clynes. 4 Serum-Free Media, Joanne Keenan, Paula Meleady, and Martin Clynes. 5 Screening Culture Media and Sera for Endotoxin Testing, Joanne Keenan and Martin Clynes. 6 Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry, Elizabeth Moran, Annemarie Larkin, Allan Masterson, and Martin Clynes. 7 The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus, Olivia Flynn and Dermot Walls. 8 Primary Culture, Finbar O Sullivan, Paula Meleady, Shirley McBride, and Martin Clynes. 9 Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes, Mark Jackman. 10 Methods for Studying Phosphoinositide Metabolism in Cultured Cells, Conor Duffy, Ailish Hynes, and Leo Quinlan. 11 Analysis of Cell Cycle and Cell Death Mechanisms, S. Verhaegen, Seamus Coyle, Lisa M. Connolly, Colette OLoughlin, and Martin Clynes. 12 Assessing Release of Secretory Products from Individual Cells, Domenico Bosco and Paolo Meda. 13 The Granulocyte/Macrophage Colony-Forming Unit Assay, Augusto Pessina. 14 Investigations into Cell Differentiation Using Cells in Culture, Shirley McBride and Martin Clynes. 15 Cell Culture Models for Mononuclear Phagocytes, Philippe Poindron and Yves Lombard. 16 The Human Leukemia Cell Line Hl-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses, Faustino Mollinedo, Antonio Santos-Beneit, and Consuelo Gajate. 17 Cell Culture Models for Polarized Epithelial Monolayers, Alan W. Baird and Ena S. Prosser. 18 Ion Channels and Cell Signaling in Cell Cultures, Valrie Urbach, Deirdre Walsh, Maria Higgins, Isabelle Leguen, Christina Doolan, John Cuffe, Elizabeth Horwitz, Catherine Halligan, Ruth Gleeson, Anthony Cullinane, and Brian Harvey. 19 Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells, Paula Meleady, Finbar O Sullivan, Shirley McBride, and Martin Clynes. 20 Culture Systems for Hepatocytes for Use in Toxicology and Differentiation Studies, Guenady Ilyin, Anne Corlu, Pascal Loyer, and Christiane Guguen-Guillouzo. 21 Cellular Models for In Vitro Toxicity Testing, Flavia Zucco, Isabella De Angelis, and Annalaura Stammati. 22 Miniaturized In Vitro Methods in Toxicity Testing, Robert O'Connor, Mary Heenan, Conor Duffy and Martin Clynes. 23 Investigation of Anti-tumor Drug Accumulation and Efflux in Cell Culture, Irene Cleary and Martin Clynes. 24 Current Status of Genetically Engineered In Vitro Systems for Biotransformation, Johannes Doehmer. 25 Scale-Up of Animal Cell Cultures, Eunan McGlinchey, D. ODriscoll and Martin Clynes. 26 Purification of Monoclonal Antibodies Produced by Animal Cells in Culture, Conor ODea, Suzanne OConnor, and Martin Clynes. 27 Biosafety Assessment of Cultured Animal Cells Used for Biopharmaceutical Production, Carl N. Martin and David W. Birch. 28 Cytokine Cell Culture Assays, Alice Redmond, Jacqueline Quinn, and Susan Molloy. 29 Antisense Methods in Cell Culture, Daragh Byrne, Carmel Daly, and Martin Clynes. 30 Transfection with Ribozymes to Investigate the Role of Specific Gene Expression, Carmel Daly, Daragh Byrne, and Martin Clynes. 31 Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture, Noel Daly and Martin Clynes. 32 Analysis of Specific and Differentially Expressed mRNAs in Cell Culture, Toni ODoherty, Risn Nic Amhlaoibh, Martin Clynes. 33 Human Gene Therapy: Review and Outlook, Gerard J. McGarrity