Animal Cell Culture: Principles and Practice

ISBN-13: 9783031194849 / Angielski / Twarda / 2023 / 327 str.

Shalini Mani; Manisha Singh; Anil Kumar

Opis

This introductory guide provides novice researchers and lab students with a thorough step-by-step approach to standard animal cell culture techniques. Coverage includes lab safety and best practices, sterility management, preparation, ethical considerations, and troubleshooting for common pain points. This is an up-to-date, indispensable handbook for early-career researchers and students, as well as established scientists in biotechnology, cell and developmental biology, pharmaceutical toxicology, cytogenetics, and more. 

Spis treści

1. Overview to animal cell culture

2. Cell culture laboratory

3. Good lab practices and bio-safety measures

4. Managing sterility in the lab

5. Media and buffer preparation for cell culture

6. Properties of cultured cells and selection of culture media

7. Selection and maintenance of cultured cells

8. Inoculation and passaging of adherent cells

9. Inoculation and passaging of suspension cells

10. Counting of cells

11. Cryopreservation of cell lines

12. Resuscitation of frozen cell line

13. Isolation and culturing of cells from different tissues

14. Differentiation, transformation and immortalization of cells

15. Co-culture techniques

16. 3D cell culture techniques

17. Stem cell culture techniques

18. Identification and removal of biological contamination in the media and cell suspensions

19. Analysis of cell growth kinetics in suspension and adherent types cell line

20. In vitro cytotoxicity analysis: mtt/xtt, trypan blue exclusion

21. Applications of animal cell culture based assays:

a. Biomarker Identification

b. Genetic Manipulation

c. Cellular development and Differentiation

d. Pathological studies

e. Pharmaceutical studies

f. Stem cell Research

g. Hybridoma Technology

22. Downstream processing of therapeutically important proteins from cultured cells

23. Ethical issues in animal cell culture

24. Common troubleshooting methods in cell culture techniques

O autorze

Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India.