Animal Cell Culture Essential Methods

ISBN-13: 9780470666586 / Angielski / Miękka / 2011 / 376 str.

John Davis

Opis

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.
This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Recenzje

"It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques." (Doody′s, 13 January 2012)

Spis treści

Contributors.

Preface.

Abbreviations.

Protocols.

1 The Cell Culture Laboratory (Sue Clarke and Janette Dillon).

2 Sterilization (Peter L. Roberts).

3 Microscopy of Living Cells (Colin Gray and Daniel Zicha).

4 Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John M. Davis).

5 Development and Optimization of Serum– and Protein–free Culture Media (Stephen F. Gorfien and David W. Jayme).

6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A. Fleck).

7 Primary Culture of Specific Cell Types and the Establishment of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris and Jan–Thorsten Schantz).

8 Cloning (John Clarke, Alison Porter and John M. Davis).

9 The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination (Peter Thraves and Cathy Rowe).

10 Systems for Cell Culture Scale–up (Jennifer Halsall and John M. Davis).

11 Good Laboratory Practice in the Cell Culture Laboratory (Barbara Orton).

Index.

Colour plates.

O autorze

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

Blurb

Animal Cell Culture: Essential Methods is an up–to–date guide to all the core concepts and techniques central to the performance of cell culture in the modern laboratory. Every chapter provides selected tried–and–tested protocols for immediate use at the bench along with a plethora of good practical advice, whilst also giving the essential background and theory, and discussing the merits and limitations of the various approaches. It represents the most accessible introduction available to the culture and manipulation in vitro of animal cells.

The book is an essential laboratory manual for anyone in academia or industry who is about to embark on cell culture work, yet it will be no less useful to the experienced worker as an easy reference guide to established approaches and techniques.

• Accessible and up–to–date guide to a broad range of methods in animal cell culture.
• Explains clearly and precisely how to carry out selected techniques in addition to giving background information on the various approaches.
• Chapters are written by leading international authorities in the field and cover both well–known and new, tried and tested, methods for working in animal cell culture.
• An essential laboratory manual for students, researchers, and industrial scientists.
• Covers cryopreservation, the design and use of serum– and protein–free media and systems for culture scale–up.