Animal Cell Biotechnology: Methods and Protocols

ISBN-13: 9781627039635 / Angielski / Miękka / 2014 / 512 str.

Ralf Portner

Opis

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Spis treści

Animal Cell Biotechnology - Methods and Protocols Editor: Ralf Pörtner Contents Preface Contributors Part I. Basic techniques for establishing production cell lines 1 Transduction Technologies David A. Fluri and Martin Fussenegger 2 Cells for gene therapy and vector production C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten 3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier 4 DNA Fingerprinting and Characterisation of Animal Cell Lines Glyn N. Stacey, Ed Byrne and J. Ross Hawkins Part II. Basic cultivation techniques 5 Microcarrier Cell culture Technology Gerald Blüml 6 Cell Encapsulation Holger Hübner 7 Tools for High-Throughput Medium and Process Optimisation Martin Jordan and Nigel Jenkins Part III. Cell characterisation and analysis 8 Cell counting and viability measurements Michael Butler and Maureen Spearman 9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai 10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors André A. Neves and Kevin M. Brindle 11 Methods for off-line analysis of nutrients and products in mammalian cell culture Heino Büntemeyer 12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation Derek Adams, Rashmi Korke, and Wei-Shou Hu 13 Measurement of apoptosis in cell culture Adiba Ishaque1 and Mohamed Al-Rubeai 14 Metabolic Flux Estimation in Mammalian Cell Cultures Chetan T. Goudar, Richard Biener, JamesM. Piret and Konstantin B. Konstantinov Part IV. Special cultivation techniques 15 Disposable bioreactors for inoculum production and protein expression Regine Eibl and Dieter Eibl 16 Hollow Fibre Cell Culture John M. Davis 17 Cultivation of mammalian cells in fixed bed reactors Ralf Pörtner and Oscar B.J. Platas Barradas 18 Configuration of Bioreactors Dirk E. Martens and Evert Jan van den End Part V. Downstream techniques 19 Membrane filtration in animal cell culture Peter Czermak, Dirk Nehring and Ranil Wickramasinghe 20 Chromatographic techniques in the downstream processing of (recombinant) proteins Ruth Freitag Part VI. Special applications 21 Vaccine production - state of the art and future needs in upstream processing Yvonne Genzel and Udo Reichl 22 Retrovirus production and characterization Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves 23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production Sabine Geisse Index

O autorze

Priv.-Doz. Dr.-Ing. Ralf Pörtner, geb. 1958 in Melle. 1978-1984 Studium der Chemietechnik in Dortmund. 1989 Promotion Uni Dortmund, Mechanische Verfahrenstechnik. 1989-1990 Post-Doc Uni Tsukuba, Japan. Seit 1990 Oberingenieur und Leiter Arbeitsgruppe Zellkulturtechnik und Tissue Engineering, TU Hamburg-Harburg, Institut für Bioprozess- und Biosystemtechnik. 2002-2007 Lehrbeauftragter HAW Hamburg. Seit 2008 Lehrbeauftragter an der FH Gießen-Friedberg. 1997 Habilitation und Ernennung zum Privatdozenten. Mitkoordinator des Forschungsschwerpunktes Regeneration, Implantate und Medizintechnik der TU Hamburg-Harburg und Sprecher der Forschungsgruppe Tissue Engineering. Studiengangkoordinator Bioverfahrenstechnik. Etwa 60 referierte Arbeiten sowie über 120 Tagungsbeiträge zu den Themen Zellkulturtechnik, Steuerung von Bioreaktoren, Tissue Engineering.