Animal Cell Biotechnology: Methods and Protocols

ISBN-13: 9781627037327 / Angielski / Twarda / 2013 / 498 str.

Ralf Portner

Opis

Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Spis treści

Part I. Cell Line Development and Selection

 

1. High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter

            Wong Cheng Lee, Ali Asgar. S. Bhagat, and Chwee Teck Lim

 

2. Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology

João Vidigal, Fabiana Fernandes, Ana S Coroadinha, Ana P Teixeira, and Paula M. Alves

                                               

3. Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine

Lukas Fliedl and Christian Kaisermayer

                                               

4. Scalable Transient Protein Expression

Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones, and Jon Zmuda

 

5. DNA Profiling and Characterisation of Animal Cell Lines

Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins

 

 

Part II. Techniques for Process Development

 

6. Tools for High-Throughput Process and Medium Optimization

Martin Jordan and Matthieu Stettler

                                   

7. Designing Media for Animal Cell Culture – CHO Cells, The Industrial Standard 

Karlheinz Landauer

 

8. Feed Optimization in Fed-Batch Culture

Ana Rita Costa, Maria Elisa Rodrigues, Mariana Henriques, Rosário Oliveira, and Joana Azeredo

                                               

9. Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches

Zhihua Xiao, Michelle Sabourin, Graziella Piras, and Stephen Gorfien

 

10. Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins        

Maria Elisa Rodrigues, Ana Rita Costa, Mariana Henriques, Joana Azeredo, and Rosário Oliveira

 

11. Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System

Steve R.C. Warr

 

 

Part III. Cell Characterisation and Analysis

           

12. Monitoring Cell Growth, Viability and Apoptosis

Michael Butler, Maureen Spearman, and Katrin Braasch

 

13. Metabolic Flux Estimation in Mammalian Cell Cultures

Chetan T. Goudar, Richard K. Biener, James M. Piret, and Konstantin B. Konstantinov

 

14. Quenching Methods for the Analysis of Intracellular Metabolites

Judith Wahrheit and Elmar Heinzle

 

15. NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors

Nelly Aranibar and Michael D. Reily

 

16. 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells

Tiago M. Duarte, Nuno Carinhas, Ana Carina Silva, Paula M. Alves, and Ana P. Teixeira

 

 

Part IV. Cultivation Techniques

           

17. Microfluidic Perfusion Culture

Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori

 

18. Disposable Bioreactors for Inoculum Production and Protein Expression          Regine Eibl, Christian Löffelholz, and Dieter Eibl

                                               

19. Configuration of Bioreactors

Dirk E. Martens, E. J. van den End, and Mathieu Streefland

                                   

20. Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes – Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)

Damir Druzinec, Katja Weiss, Christiane Elseberg, Denise Salzig, Matthias Kraume, Ralf Pörtner, and Peter Czermak

 

21. Cell Assessment by Atline Microscopy

Alexander Babitzky, Patrick Lindner, and Thomas Scheper

 

22. Seed Train Optimization for Cell Culture

Björn Frahm

 

 

Part V. Special Applications

 

23. Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines   

Yvonne Genzel, Jana Rödig, Erdmann Rapp, and Udo Reichl

           

24. Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles

Heike Cremer, Ingrid Bechtold, Marion Mahnke, and René Assenberg

 

25. Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology

            Ruth Freitag

 

26. Virus Separation Using Membranes        

Tanja A. Grein, Ronald Michalsky, and Peter Czermak

O autorze

Priv.-Doz. Dr.-Ing. Ralf Pörtner, geb. 1958 in Melle. 1978-1984 Studium der Chemietechnik in Dortmund. 1989 Promotion Uni Dortmund, Mechanische Verfahrenstechnik. 1989-1990 Post-Doc Uni Tsukuba, Japan. Seit 1990 Oberingenieur und Leiter Arbeitsgruppe Zellkulturtechnik und Tissue Engineering, TU Hamburg-Harburg, Institut für Bioprozess- und Biosystemtechnik. 2002-2007 Lehrbeauftragter HAW Hamburg. Seit 2008 Lehrbeauftragter an der FH Gießen-Friedberg. 1997 Habilitation und Ernennung zum Privatdozenten. Mitkoordinator des Forschungsschwerpunktes Regeneration, Implantate und Medizintechnik der TU Hamburg-Harburg und Sprecher der Forschungsgruppe Tissue Engineering. Studiengangkoordinator Bioverfahrenstechnik. Etwa 60 referierte Arbeiten sowie über 120 Tagungsbeiträge zu den Themen Zellkulturtechnik, Steuerung von Bioreaktoren, Tissue Engineering.