Angiogenesis

ISBN-13: 9781071620588 / Angielski / Twarda / 2022

Opis

This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research. For example, this includes protocols to explore how transcriptional phenotyping can be achieved, both in terms of exploring tissue-specific heterogeneity to the use of database mining of existing datasets. Similarly, bioinformatic techniques run alongside protocols for transcript analysis, cell biology, pathology, as well as physiological and developmental angiogenesis models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Angiogenesis: Methods and Protocols provides a meaningful compendium of practical approaches to angiogenesis and endothelial cell biology research.

Spis treści

Part I: Developmental Biology

 

1. The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo

            Gabriela D’Amico and Christiana Ruhrberg

 

2. A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy

            Yan Chen, Paul C. Evans, and Robert N. Wilkinson

 

3. Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling

            Lachlan Whitehead, Emma C. Watson, and Zoe L. Grant

 

4. Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation

            Elena Ioannou and Christiana Ruhrberg

 

5. Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina

            Alessandro Fantin

 

Part II: Adult Physiology and Pathology

 

6. Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin

            Nikita Ved and Jacinta Isabelle Kalisch-Smith

 

7. Analyzing Lymphatic Vessel Patterning in Adult Tissue

            Louise A. Johnson

 

8. Transmission Electron Microscopy of Endothelium

            Zubair Ahmed Nizamundeen, Loubna ElSaboni, Grace Vetch, Jumana Karim, Chris R. Neal, Claire L. Allen, David O. Bates, and Kenton P. Arkill

 

9. Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice

            Sohni Ria Bhalla, Federica Riu, Maria J.C. Machado, and David O. Bates

 

10. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts

            Peter Baluk and Donald M. McDonald

 

11. Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence

            Naseeb K. Malhi, David O. Bates, Kenton P. Arkill, and Claire L. Allen

 

12. An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery

            Dima Majbour, Ariana D. Suarez-Martinez, Nicholas A. Hodges, Arinola O. Lampejo, Banks M. Lomel, Elijah W. Rice, Hulan Shang, Adam J. Katz, and Walter L. Murfee

 

13. Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart

            Konstantinos Klaourakis, Paul R. Riley, and Joaquim Miguel Vieira

 

14. Quantifying Lymphatic Vessel Density in Human Tissue Samples

            Stewart G. Martin, Emad Rakha, and Sarah J. Storr

 

15. Quantifying Vascular Remodeling in the Mouse Spinal Cord

            M.E. Da Vitoria Lobo, Lydia Hardowar, and Richard P. Hulse

 

16. Quantitative Methods to Assess Adipose Vasculature

            Martina Rudnicki, Alexandra Pislaru, and Tara L. Haas

 

17. Quantification of Angiogenesis in Laser Choroidal Neovascularization

            Kathryn R. Green, Nicholas Beazley-Long, Amy P. Lynch, Claire L. Allen, David O. Bates, and Andrew V. Benest

 

Part III: Reducing Complexity: Growing and Studying Endothelial Cells Outside the Body

 

18. A Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue

            Jacqueline Herold and Joanna Kalucka

 

19. Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides

            Zarah B. Tabrizi, Kathryn R. Green, Amy P. Lynch, Nada S. Ahmed, Nicholas Beazley-Long, and Andrew V. Benest

 

20. Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells

            Philip Kitchen, Kevin Gaston, and Padma-Sheela Jayaraman

 

21. In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis

            Adam F. Odell and Aarren J. Mannion

 

22. Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates

            Joshua H. Bourne

 

23. Isolation of Cardiac Endothelial Cells for Transcriptomic Analysis of the Zebrafish and Mouse Heart

            Ziwen Li, Katherine M. Ross Stewart, Finnius A. Bruton, Martin A. Denvir, and Mairi Brittan

 

24. The Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces

            Hannah A. Strobel and James B. Hoying

 

25. Endothelial Cell Fibrin Gel Angiogenesis Bead Assay

            Eva Clavane, Hannah Taylor, Richard Cubbon, and Paul Meakin

 

26. Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers

            Aarren J. Mannion

 

27. Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications

            Kavita Raniga, Nguyen T.N. Vo, and Chris Denning

 

Part IV: Making Use of Publically Existing Datasets and Resources

 

28. Finding and Verifying Enhancers for Endothelial-Expressed Genes

            Alice Neal, Helena Rodriguez-Caro, and Sarah De Val

 

29. Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching

            Guillermo Díez Pinel, Joseph L. Horder, John R. King, Alan McIntyre, Nigel P. Mongan, Gonzalo Gómez López, and Andrew V. Benest