Androgen Action: Methods and Protocols

ISBN-13: 9781493962013 / Angielski / Miękka / 2016 / 389 str.

Fahri Saatcioglu

Opis

Androgen Action provides a tool box to study various phases of androgen action, from its entry to the cell, to the phenotypic response that the cell mounts. The text includes up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists.

Recenzje

From the reviews:

"The Editor asked more than 50 experts from three continents and put them together to contribute with their up-to-date written chapters. This valuable volume begins with two chapters on the history of androgen research, especially with androgen action and prostate carcinogenesis. ... This volume will certainly provide the reader with a comprehensive overview of, and practical guidance on, the discrete methodologies which are propelling androgen research forward." (Endocrine Regulations, October, 2012)

"This book details protocols to determine androgen levels, actions mediated by AR, and cross-talk with other key signaling networks. ... The techniques are most appropriate for biochemists and molecular and cellular biologists who are engaged in the study of androgen-mediated events. ... This effectively written and thought-out book will serve as useful resource to decipher the complex actions of androgens. ... The compilation of knowledge and wisdom in this book will be beneficial to current and future researchers." (Clay E. S. Comstock, Doody's Book Reviews, February, 2012)

Spis treści

Part I. Reviews

 

1. Molecular Mechanisms of Androgen Action – A Historical Perspective

            Albert O. Brinkmann

 

2. Androgen Action during Prostate Carcinogenesis

            Diping Wang and Donald J. Tindall

 

Part II. Analysis of Androgens

 

3. Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry

            Mark Titus and Kenneth B. Tome

 

4. Ligand Competition Binding Assay for the Androgen Receptor

            Clémentine Féau, Leggy A. Arnold, Aaron Kosinski, and R. Kiplin Guy

 

Part III. Androgen receptor biology

 

5. Analysis of Androgen Receptor Activity by Reporter Gene Assays

            Harri Makkonen, Tiina Jääskeläinen, Miia Rytinki, and Jorma J. Palvimo

 

6. Identification and Characterization of Androgen Response Elements

            Reinhilde De Bruyn, Rita Bollen, and Frank Claessens

 

7. In vitro and In vivo Silencing of the Androgen Receptor

            Helen Cheng, Eric Leblanc, and Paul S. Rennie

 

8. Analysis of Interdomain Interactions of the Androgen Receptor

            Elizabeth M. Wilson

 

9. Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells

            Hatice Zeynep Kirli and Fahri Saatcioglu

 

10. FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells

            Fred Schaufele

 

Part IV. Analysis of Androgen Receptor Modifications and Interactions

 

11. Analysis of Nuclear Receptor Acetylation

            Chenguang Wang, Michael Powell, Lifeng Tian, and Richard G. Pestell

 

12. Analysis of Androgen Receptor SUMOylation

            Miia M. Rytinki, Sanna Kaikkonen, Päivi Sutinen, and Jorma J. Palvimo

 

13. Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor

            Claudia Gerlach, Daniela Roell, and Aria Baniahmad

 

14. Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF

            Thomas Linke, Martin Scholten, and Aria Baniahmad

 

Part V. Recent Advances in Studying Androgen Receptor Signalling

 

15. Global Identification of Androgen Response Elements

            Charles E. Massie and Ian G. Mills

 

16. Tissue Specific Knockout of Androgen Receptor in Mice

            Tzuhua Lin, Shuyuan Yeh, and Chawnshang Chang

 

17. Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting

            Holly M. Nguyen and Eva Corey

 

18. Automated Microscopy and Image Analysis for Androgen Receptor Function

            Sean M. Hartig, Justin Y. Newberg, Michael J. Bolt, Adam T. Szafran, Marco Marcelli, and Michael A. Mancini

 

Part VI. Cross-talk of Androgen and Other Signalling Pathways

 

19. Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer

            Delila Gasi and Jan Trapman 

 

20. Regulation of Apoptosis by Androgens in Prostate Cancer Cells

            Yke Jildouw Arnoldussen, Ling Wang, and Fahri Saatcioglu

 

21. Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association

            Antimo Migliaccio, Gabriella Castoria, and Ferdinando Auricchio

 

22. Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells

            Jørgen Sikkeland, Torstein Lindstad, and Fahri Saatcioglu

Blurb

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states.