Ancient DNA Typing: Methods, Strategies and Applications

ISBN-13: 9783642077050 / Angielski / Miękka / 2010 / 298 str.

Susanne Hummel

Opis

Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.

Recenzje

From the reviews:

"This is a book that does what it says on the cover. Written by one of the leaders in the aDNA field, it covers both the basic techniques and the applications of aDNA in archaeological, anthropological and zoological studies. Susanne Hummel's writing is clear, she has chosen excellent diagrams to illustrate her text, and she includes many references to help the reader find their way into the primary literature. On the whole, she is to be congratulated for producing such a comprehensive guide ... ." (Terence A. Brown and Keri A. Brown, Human Genetics, Vol. 114 (1), 2003)

"This book is a valuable introduction to the field of ancient DNA analysis and of direct relevance to the forensic community. Many of the problems and challenges that the author addresses are identical to those increasingly being faced by forensic biologists ... . In this respect the great value of the book is its extensive descriptions of the methodologies and strategies ... . I strongly recommend Ancient DNA Typing as a valuable reference source to anyone required to push current DNA testing methods ... ." (Peter R Gunn, Australian Journal of Forensic Science, Vol. 35, 2003)

Spis treści

1 Introduction.- 2 DNA markers.- 3 aDNA extraction.- 4 PCR.- 5 Analysis of PCR products.- 6 Authenticity of results.- 7 Applications.- 8 Protocols.- I Laboratory equipment.- I.1 Pre-PCR laboratory equipment.- I.2 PCR laboratory.- I.3 Post-PCR laboratory.- I.4 Photographic documentation.- II PCR trouble shooting.- III Internet sites of interest.