Ancestral Sequence Reconstruction

ISBN-13: 9780199299188 / Angielski / Twarda / 2007 / 272 str.

David A. Liberles

Opis

Ancestral sequence reconstruction is a technique of growing importance in molecular biology and comparative genomics. As a powerful technique for both testing evolutionary and ecological hypotheses as well as uncovering the link between sequence and molecular phenotype, there are potential applications in a number of fields. Beginning with a historical overview of the field including apllications, the discussion then moves into potential applications in drug discovery and the pharmaceutical industry. A section on computational methodology provides a detailed discussion on available methods for reconstructing ancestral sequences, including advantages, disadvantages, and potential pitfalls. Purely computational applications, including whole proteome reconstruction are discussed. Another section provides a detailed discussion on taking computationally reconstructed sequences and synthesizing them in the laboratory, while the last section describes scientific questions where experimental ancestral sequence reconstruction coupled to a computaional and experimental how-to guide, while simultaneously addressing some of the hot topics in the field.

Recenzje

This combination of theory and application is unusual and broadens the appeal of the book for researchers or for teachers of advanced seminars...This gives the book a light and readable quality

O autorze

David A. Liberles is Assistant Professor at the Department of Molecular Biology, University of Wyoming, USA