Anaerobiosis and Stemness: An Evolutionary Paradigm for Therapeutic Applications

ISBN-13: 9780128005408 / Angielski / Twarda / 2015 / 326 str.

Ivanovic, Zoran Vlaski-Lafarge, Marija

Opis

Anaerobiosis and Stemness: An evolutionary paradigm provides a context for understanding the many complexities and evolutionary features of stem cells and the clinical implications of anaerobiosis stem cells. Combining theoretical and experimental knowledge, the authors provide a broad understanding of how the absence or low concentration of oxygen can play an influential role in the maintenance and self-renewal of stem cells and stem cell differentiation. This understanding has clinical implications for the fields of regenerative medicine, cancer biology and transplantation, as well as cell engineering and cell therapy. Anaerobiosis and Stemness is an important resource for stem cell and developmental biologists alike, as well as oncologists, cancer biologists, and researchers using stem cells for regeneration.

• Highlights the molecular and evolutionary features of stem cells which make them so important to all biological research
• Explores methods of isolation, characterization, activation, and maintenance of stem cells
• Includes models for clinical application in regenerative medicine, cancer therapy, and transplantation

Recenzje

"...provides the key for understanding and predicting stem cell behavior in vivo and ex vivo, which is of paramount importance for the cell engineering and anti-cancer strategies." --Serbian Archives of Medicine

"The "evolutionary stem cell paradigm”of Z. Ivanovic and M. Vlaski provides the key for understanding and predicting stem cell behavior in vivo and ex vivo, which is of paramount importance for the cell engineering and anti-cancer strategies. The concept of anaerobiosis and stemness presented in this unique book implies the epigenetic missing link in stem cells behavior. The evolutionary paradigm described, although partly hypothetical, is important for understanding developmental biology." --Professor Pavle Milenkovic, MD, PhD, President of the Academy of Medical Sciences, Serbian Medical Society

Spis treści

TABLE OF CONTENTS:PREFACESPECIAL REMARKS1) What entity could be called a "stem cell”?2) In situ "normoxia” vs. "hypoxia”Part I:ANAEROBIOSIS AND STEM CELL ENTITY1) The "hypoxic” stem cell niche2) Low O2 concentrations and the maintenances of stem cells ex vivo 3) Quiescence/proliferation issue and stem cell niche4) Metabolic peculiarities of the stem cell entity: energetic metabolism and oxidative status5) "Hypoxic” signaling, metabolic type, quiescence, self-renewal and differentiation

Part II:ANAEROBIC-TO-AEROBIC EUKARYOTE EVOLUTION - A PARADIGM FOR STEM CELL SELF-RENEWAL, COMMITMENT AND DIFFERENTIATION?1) Evolution of eukaryotes with respect to the appearance and rise of oxygen in the atmosphere: anaerobiosis, facultative aerobiosis and aerobiosis 2) Evolution of mitochondria in eukaryotes and mitochondria "maturing” from stem cells to committed progenitors and mature cells3) Evolutionary origins of stemness: relationship between self-renewal and ancestral eukaryote biology; conservation of self-renewal principle in parallel with the adaptation to O24) Metabolic and genetic features of ancestral eukaryotes vs. metabolism and "master pluripotency genes” of stem cells5) Some other features concerning the analogy "stem cells - primitive eukaryotes": ABC transporters' anaerobiosis/stemness link6) Harnessing anaerobic nature of stem cells for the use in regenerative medicine 7) Cancer stem cell case and evolutionary paradigm

O autorze

Zoran Ivanovic is Scientific Director of French Blood Institute for the regions of Aquitaine and Limousin (Bordeaux, France) and Head of R&D Cell Engineering Research Laboratory. He is also the Group Leader ("Adult stem cells”) in UMR 5164 CNRS/University of Bordeaux. He received his MD, MSc (system and comparative physiology) and DSc (experimental hematology) degrees at Belgrade University, and the highest French Degree "HDR” (Habilitation à Diriger les Recherches) at Bordeaux 2 University. He specialized in Transfusion (Bordeaux 2 University) and in Cell Therapy (Paris 7 University). Research Professor at Institute for Medical Research of Belgrade University, former Associate Professor of Hematology at Limoges and Bordeaux Universities (1999-2002), he has been, since 2011, guest professor at the Medical Faculty of Nis University. Dr Ivanovic obtained several grants in the field of cord blood and adult stem cell research. His group studies the anaerobic proliferation of stem cells and develops clinical-scale ex-vivo expansion procedures, pre-conditioning of stem cells for transplantation as well as new approaches of stem and progenitor cell conservation in hypothermia. He published 123 articles and book chapters, realized 150 meeting communications and supervised several PhD and master theses. He serves as Academic Editor of PLoS One as well as reviewer for many scientific journals. Marija Vlaski Lafarge is staff scientist in R&D Cell Engineering Research Laboratory of French Blood Institute for the regions of Aquitaine and Limousin (Bordeaux, France) and in UMR 5164 CNRS/University of Bordeaux. She obtained her PhD degree at Faculty of Sciences (Cell physiology), University of Belgrade, Serbia. Her scientific activity is devoted to stem cell metabolism and physiology studies. Dr. Vlaski Lafarge presented her work in articles, book chapters and meeting communications. She participated in the grants preparation, reviewed several scientific manuscripts, and governed many student's research projects.