Alcohol: Methods and Protocols

ISBN-13: 9781617378379 / Angielski / Miękka / 2010 / 420 str.

Laura E. Nagy

Opis

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics.

Spis treści

Contents Preface Contributors Part I. Animal Models of Acute and Chronic Ethanol Exposure Acute models of ethanol exposure to mice Timothy P. Plackett and Elizabeth J. Kovacs A voluntary oral-feeding rat model for pathological alcohol liver injury George L. Tipoe, Emily C. Liong, Tung-Ming Leung,and Amin A. Nanji Intragastric ethanol infusion model in rodents Hide Tsukamoto, Hasmik Mikrtchyan and Alla Dynnyk A practical method of chronic ethanol administration in mice Ruth A Coleman, Betty M. Young, Lucas E. Turner and Robert T. Cook Part II. Animal models of ethanol exposure during development Analysis of ethanol developmental toxicity in Zebrafish Robert L. Tanguay and Mark J. Reimers The avian embryo in fetal alcohol research Susan M. Smith Artificial rearing Hector D. Dominguez and Jennifer D. Thomas Intragastric intubation of alcohol during the perinatal period Sandra J. Kelly and R. Charles Lawrence Part III. Cell culture approaches to studying ethanol exposure 9. Human monocytes, macrophages and dendritic cells – alcohol treatment methods Gyongyi Szabo and Pranoti Mandrekar 10. Generation and use of primary rat cultures for studies of the effects of ethanol Amanda Lindke, Barbara Tremper-Wells and Michael W. Miller 11. Development and properties of HepG2 cells which constitutively express CYP2E1 Defeng Wu and Arthur I. Cederbaum 12. Modeling the impact of alcohol on cortical development in a dish: Strategies from mapping neural stem cell fate Rajesh C. Miranda, Daniel R. Santillano, Cynthia Camarillo, and DouglasDohrman Part IV. Regulation of Specific Organ Systems in Response to Ethanol 13. Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayer R.K. Rao 14. Alcohol-induced oxidative stress in the liver: in vivo measurements Gavin E. Arteel 15. Isolation of Kupffer cells from rats fed chronic ethanol Megan R. McMullen, Michele T. Pritchard and Laura E. Nagy 16. Dendritic cells in chronic in vivo ethanol exposure models Kevin L. Legge and Annette J. Schleuter 17. Formation and immunological properties of aldehyde-derived protein adducts following alcohol consumption Geoffrey M. Thiel, Lynell W. Klassen and Dean J. Tuma 18. Assessment of natural killer (NK) and NKT cells in murine spleens and livers Michael R. Shey and Zuhair K. Ballas 19. Polyclonal and antigen-specific responses of T cells and T cell subsets Betty M. Young, Susan Wiechert, Ruth A. Coleman, Prajwal Gurung, and Robert T. Cook 20. B Cell studies and chronic ethanol mice Shilpi Verma, Carla-Maria A. Alexander, Michael J. Carlson, Lorraine T. Tygrett and Thomas J. Waldschmidt 21. Histological analysis of bone Urszula T. Iwaniec, Thomas J. Wronski and Russell T. Turner 22. Assessing effects of alcohol consumption on protein synthesis in striated muscles Thomas C. Vary and Charles H. Lang 23. Methods to investigate the effects of chronic ethanol on adipocytes Becky M. Sebastian, Li Kang, Xiaocong Chen, and Laura E. Nagy Part V. Proteomic and Genomic Approaches to Study Responses to Ethanol 24. Proteomic approaches to identify and characterize alterations to the mitochondrial proteome in alcohol liver disease Shannon M. Bailey, Kelly K. Andrina, Aimee Landar, and Victor M. Darley-Usmar 25. Alcoholic liver disease and the mitochondrial ribosome

Blurb

Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics. As part of the successful Methods in Molecular Biology™ series, each chapter provides step-by-step laboratory protocols, lists of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls.

Comprehensive and highly specific, Alcohol: Methods and Protocols strives to further spur investigators to delve into the complex and fascinating processes of the adaptive and maladaptive responses humans have developed to the consumption of alcohol.