Aging: Methods and Protocols

ISBN-13: 9781071605912 / Angielski / Twarda / 2020 / 277 str.

Sean P. Curran

Spis treści

. Measuring the Replicative Lifespan of Saccharomyces cerevisiae using the HYAA Microfluidic Platform

Ruofan Yu, Myeong Chan Jo, and Weiwei Dang

 

2. Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches

Adam B. Cornwell and Andrew V. Samuelson

 

3. Simple Culture Apparatus for Individual C. elegans Culture

William E Pittman, Drew B Sinha, Holly E Kinser, Nisha S Patil, Eric S Terry, Isaac B Plutzer, Julia Hong, and Zachary Pincus

 

4. Analysis of Drosophila melanogaster Life Span

Gary N. Landis, Devon Doherty, and John Tower

 

5. Assessment of Mouse Fitness as Determined through Treadmill Running and Walking

Joseph C. Reynolds and Changhan Lee

 

6. Human Population Genetics in Aging Studies for Molecular Biologists

Brendan Miller, Amin Haghani, Jennifer Ailshire, and T. Em Arpawong

 

7. Design and Analysis of Pharmacological Studies in Aging

Khalyd J. Clay and Michael Petrascheck

 

8. Methods for Assessing Fertility in C. elegans from a Single Population

Chia-An Yen and Sean P. Curran

 

9. Metabolic Assessment of Lipid Abundance and Distribution

James D. Nhan and Sean P. Curran

 

10. Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography-Mass Spectrometry

Elizabeth C. Pino and Alexander A. Soukas

 

11. Assessing Insulin and Glucose Tolerance in the Aging Mouse

Roberta Buono and Sebastian Brandhorst

 

12. High-throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome

Fan Zhang, Jessica L. Weckhorst, Adrien Assié, Anastasia S. Khodakova, Mario Loeza-Cabrera, Daniela Vidal, and Buck S. Samuel

 

13. Measurements of Innate Immune Function in C. elegans

Kyle J. Foster, Deborah L. McEwan^, and Read Pukkila-Worley

 

14. Measuring Phagocytosis in Bone Marrow-Derived Macrophages and Peritoneal Macrophages with Aging

 

Ryan Lu, Nirmal K. Sampathkumar, and Bérénice A. Benayoun

 

15. Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells

Mintie Pu and Siu Sylvia Lee

 

16. Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age

Rachel Kaletsky and Coleen T. Murphy

 

17. Measuring Tissue-specific Autophagy Flux in Adult Caenorhabditis elegans

Jessica T. Chang, Malene Hansen, and Caroline Kumsta

 

18. Assay Development and Measurement of the Aging Biomarker Humanin

Brendan Miller and Junxiang Wan

 

19. Staining and Quantification of β-amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Disease

Amy Christensen and Christian J. Pike

 

20. Multimodal Imaging of Cerebral Microhemorrhages and White Matter Degradation in Geriatric Patients with Mild Traumatic Brain Injury

Maria Calvillo, Di Fan, and Andrei Irimia

 

21. Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-based Approach 

Hongqiao Zhang, Natalie Lyn, Amin Haghani, and Henry Jay Forman

 

22. Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging

Juan Manuel Iglesias-Pedraz and Lucio Comai

 

Blurb

This volume details the impact of aging on biological system and to understand the basis of this diversity with the goal of elucidating mechanisms to optimize health. Chapters guide readers through different animal models and methodologies to examine aging at the organism, tissue, cellular, and molecular levels across the lifespan. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge,  Aging: Methods and Protocols aims to ensure successful results in the further study of this vital field.