1. Plant Tissue culture: General Introduction and Background 2. Micropropagation of tissue culture plants 3. Plant tissue culture: Role of media, nutrient, pH and other factors 4. Role of plant growth regulators in plant tissue culture 5. Plant tissue culture: somatic embryogenesis and organogenesis 6. plant tissue culture approach for embryo rescue of plants 7. Anther culture for haploid plant production 8. Plant tissue culture: Protoplast isolation, fusion and their regeneration 9. Plant tissue culture: Production of virus free plants using meristem culture 10. Genetic transformation via plant tissue culture techniques: current and future approaches 11. Somaclonal variations and clonal fidelity testing through molecular markers 12. Physiological factors impress the growth and development of tissue culture plants 13. Plant tissue culture targeting germplasm conservation 14. Role of tissue culture in pharmaceuticals and secondary metabolites production 15. Scope and opportunities of plant tissue culture for marketing and commercialization 16. Plant tissue culture: Advances and Prospective