Advances in New Technology for Targeted Modification of Plant Genomes

ISBN-13: 9781493925551 / Angielski / Twarda / 2015 / 166 str.

Feng Zhang; Holger Puchta; James Thomson

Opis

Over the past 50 years, biotechnology has been the major driving force for increasing crop productivity. Particularly, advances in plant genetic engineering technologies have opened up vast new opportunities for plant researchers and breeders to create new crop varieties with desirable traits. Recent development of precise genome modification methods, such as targeted gene knock-out/knock-in and precise gene replacement, moves genetic engineering to another level and offers even more potentials for improving crop production. The work provides an overview of the latest advances on precise genomic engineering technologies in plants. Topics include recombinase and engineered nucleases-mediated targeted modification, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. Finally, challenges and impacts of the new technologies on present regulations for genetic modification organisms (GMOs) will be discussed.

Spis treści

Table of Contents

Ch. 1: Double-strand break repair and its application to genome engineering in plants

            Holger Puchta, Friedrich Fauser

 

Ch. 2: Engineering meganuclease for precise plant genome modification

            Fayza Daboussi, Thomas J. Stoddard, Feng Zhang

 

Ch. 3: High efficient genome modification by designed Zinc Finger Nuclease

            Yiping Qi

 

Ch. 4: Engineered TAL effector proteins:  versatile reagents for manipulating

plant genomes

Michelle Christian, Daniel F. Voytas

 

Ch. 5: Oligo-mediated targeted gene editing

Greg F. W. Gocal, Christian Schöpke, Peter R. Beetham

 

Ch. 6: Gene targeting in crop species with effective selection systems

Takaki Yamauchi, Shigeru Iida

 

Ch. 7: Recombinase Technology for Precise Genome Engineering

James G. Thomson, Ann Blechl

 

Ch. 8: PBRM1: Developing CRISPR technology in major crop plants

Kunling Chen, Caixia Gao

O autorze

Feng Zhang, Research Director, Cellectis plant sciences

Holger Puchta, Professor, University of Karlsruhe

James Thompson, Principle Investigator, USDA

Blurb

This work provides an overview of the latest advances on precise genomic engineering technologies in plants. The research provided covers a wide range of topics, including recombinase and engineered nucleases-mediated targeted modification, negative/positive selection-based homologous recombination, and oligo nucleotide-mediated recombination. The text also discusses challenges and impacts of new technologies on present regulations for genetically modified organisms (GMOs).