Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids

ISBN-13: 9780081005576 / Angielski / Twarda / 2017 / 256 str.

Surendra Nimesh; Ramesh Chandra; Nidhi Gupta

Opis

Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids addresses several issues related to safe and effective delivery of nucleic acids (NAs) using nanoparticles. A further emphasis would be laid on the mechanism of delivery of NAs, the barriers encountered and the strategies adapted to combat them. An exhaustive account of the advantages as well shortcomings of all the delivery vectors being employed in delivery of various NAs will be provided. On final note the regulatory aspects of nanoparticles mediated NA would be discussed, with focus on their clinical relevance. The design and development of nucleic acid-based therapeutics for the treatment of diseases arising from genetic abnormalities has made significant progress over the past few years. NAs have been widely explored for the treatment of cancer and infectious diseases or to block cell proliferation and thereby caused diseases. Advances in synthetic oligonucleotide chemistry resulted in synthesis of NAs that are relatively stable in in vivo environments. However, cellular targeting and intracellular delivery of NAs still remains a challenge. Further development of NA-based therapeutics depends on the progress of safe and effective carriers for systemic administration. Nanomedicine has facilitated availability of vectors with diminished cytotoxicity and enhanced efficacy which are rapidly emerging as systems of choice. These vectors protect NAs from enzymatic degradation by forming condensed complexes along with targeted tissue and cellular delivery. During the past few years, a myriad reports have appeared reporting delivery of NAs mediated by nanoparticles. This book will provide an overview of nanoparticles being employed in the in vitro and in vivo delivery of therapeutically relevant NAs like DNA, siRNA, LNA, PNA, etc.

• Provides a complete overview of the applicatiosn of nanomedicine in the delivery of nucleic acids, from characterization of nanoparticles, to in vitro and in vivo studies
• Discusses delivery issues of less well explored nucleic acids, like PNAs, Ribozymes, DNAzymes, etc.
• Summarizes the current state of research in nucleic acid delivery and underscores the future of nanomedicine in this field

Spis treści

1. Nanomedicine for delivery of therapeutic molecules 2. Nanoparticles as nucleic acid delivery vectors 3. Methods for characterization of nanoparticles 4. Nanoparticles for DNA delivery 5. Nanoparticles for siRNA-mediated gene silencing 6. Nanoparticles for locked nucleic acid delivery 7. Nanoparticles for ribozymes delivery 8. Nanoparticles for DNAzymes delivery 9. Pharmacokinetics and biodistribution of the nanoparticles 10. Nanotoxicology: Evaluation of toxicity potential of nanoparticles 11. Regulatory aspects of nanoparticulate mediated nucleic acid delivery systems 12. Clinical studies and future prospects

O autorze

Dr. Surendra Nimesh is the author/co-author of two Elsevier books: The Design and Development of Novel Drugs and Vaccines (2021) and Gene Therapy: Potential Applications of Nanotechnology (2013) an internationally recognized expert of nanotechnology for biological applications with specialization in drug and gene delivery. He received his M.S. in Biomedical Science from Dr. B.R. Ambedkar Center for Biomedical Science Research (ACBR), University of Delhi, Delhi, India in 2001. He completed his PhD. in Nanotechnology at ACBR and Institute of Genomics and Integrative Biology (CSIR), Delhi, India in 2007. After completing his postdoctoral studies at Ecole Polyetchnique of Montreal, Montreal in 2009, he joined Clinical Research Institute of Montreal, Montreal, Canada as Postdoctoral Fellow. He worked for a short duration at McGill University, Montreal and thereafter joined Health Canada, Canada as NSERC visiting fellow in 2012. He joined Central University of Rajasthan, India as UGC-Assistant Professor at Department of Bitechnology, School of life Sciences in 2013. He has authored more than 20 research papers, 10 review articles in international peer reviewed journal, 8 book chapters and 3 books. His research interests include nanoparticles mediated gene, siRNA and drug delivery for therapeutics. Dr. Nidhi Gupta received her M.S. in Biomedical Science from Dr. B.R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi, Delhi, India in 2004. She did her PhD. with Dr. A.C. Banerjea at National Institute of Immunology (NII), New Delhi, India in 2008. After completing her doctoral research she joined Clinical Research Institute of Montreal, Montreal, Canada as Postdoctoral Fellow in 2008 and later worked at McGill University, Montreal. She joined The IIS University, India as Research Scientist at Department of Biotechnology in 2014. Her field of interest includes catalytic nucleic acids, siRNA and antisense.