Advanced Photon Counting: Applications, Methods, Instrumentation

ISBN-13: 9783319156354 / Angielski / Twarda / 2015 / 370 str.

Peter Kapusta; Michael Wahl; Rainer Erdmann

Opis

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Forster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Recenzje

"The book is quite specialised and will likely be mainly of interest to researchers developing new photon counting techniques, fluorescence-based or not. ... In addition to the constructors of photon counting systems, it could also be useful to some as a reference for comparing results, which could include those working in the life sciences as well as physicists and chemists." (David Birch, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)

Spis treści

Modern TCSPC electronics - Principles and Acquisition Modes

Michael Wahl 

 

Single-photon counting detectors for the visible range between 300 nm and 1000 nm

Andreas Bülter

 

Single-photon detectors for infrared wavelengths in the range 1 to 1.7 μm

Gerald S. Buller and Robert J. Collins

 

Modern pulsed diode laser sources for time-correlated photon counting

Kristian Lauritsen, Sina Riecke#, Andreas Bülter, Thomas Schönau

 

Advanced FCS: an Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual Focus FCS

Thomas Dertinger and Steffen Rüttinger

 

Lifetime-weighted FCS and 2D FLCS: Advanced application of time-tagged TCSPC

Kunihiko Ishii, Takuhiro Otosu, and Tahei Tahara

 

MFD-PIE and PIE-FI: Ways to extract more information with TCSPC

Anders Barth, Lena Voith von Voithenberg, Don C. Lamb

 

Photon Antibunching in Single Molecule Fluorescence Spectroscopy

Kristin S. Grußmayer and Dirk-Peter Herten

 

FLIM Strategies for Intracellular Sensing: Fluorescence Lifetime Imaging as a Tool to Quantify Analytes of Interest

Maria J. Ruedas-Rama, Jose M. Alvarez-Pez, Luis Crovetto, Jose M. Paredes, Angel Orte

 

Multiple-Pulse Pumping with Time-Gated Detection for Enhanced Fluorescence Imaging in Cells and Tissue

Rafal Fudala, Ryan M. Rich, Joe Kimball, Igna

cy Gryczynski, Sangram Raut,  Julian Borejdo, Dorota L. Stankowska, Raghu R. Krishnamoorthy, Karol Gryczynski, Badri P. Maliwal, Zygmunt Gryczynski

 

Pattern based linear un-mixing for efficient and reliable analysis of multi-component TCSPC-data

Ingo Gregor and Matthias Patting

 

Metal-Induced Energy Transfer

Narain Karedla, Daja Ruhlandt, Anna M. Chizhik, Jörg Enderlein, Alexey I. Chizhik

 

The importance of photon arrival times in STED microscopy

Giuseppe Vicidomini

 

Single color centers in diamond as single photon sources and quantum sensors

Boris Naydenov and Fedor Jelezko

 

Photon counting and timing in quantum optics experiments

Andreas Ahlrichs, Benjamin Sprenger, Oliver Benson

 

Photon counting in diffuse optical imaging

Dirk Grosenick

Blurb

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.