Adult Stem Cells: Methods and Protocols

ISBN-13: 9781493982936 / Angielski / Miękka / 2018 / 324 str.

Paolo Di Nardo; Sanjiv Dhingra; Dinender K. Singla

Spis treści

Preface...
Table of Contents...
Contributing Authors...

1. A Simple Protocol to Isolate, Characterize and Expand Dental Pulp Stem Cells
Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta

2. Derivation of Mesenchymal Stem cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells
Glen Lester Sequiera, Niketa Sareen, Ejlal Abu Al-Rub, and Sanjiv Dhingra

3. Compact Bone-Derived Multipotent Mesenchymal Stromal Cells (MSCs) for the Treatment of Sjogren’s-Like Disease in NOD Mice
Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D.Tran

4. Manipulating the Proliferative Potential of Cardiomyocytes by Gene Transfer
Giulia Prosdocimo and Mauro Giacca

5. Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells
Ghazaleh Hajmousa and Martin C. Harmsen

6. Isolation and In Vitro Characterization of Epidermal Stem Cells
Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen

7. Endothelial Progenitor Cells: Procedure for Cell Isolation and Characterization
Garikipati V.N. Srikanth, Zhongjian Cheng, and Raj Kishore

8. Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID
Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar

9. Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID
Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar

10. Isolation, Characterization, and Expansion of Cancer Stem Cells
Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia

11. Cardiac Progenitor Cell Isolation from Human Auricles
Paolo Di Nardo and Francesca Pagliari

12. Isolation and Culture of Satellite Cells from Mouse Skeletal Muscle
Antonio Musarò and Silvia Carosio

13. Isolation of Stromal Stem Cells from Adipose Tissue
Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone

14. Human Induced Pluripotent Stem Cell-Derived Mesencymal Stem Cells as an Individual-Specific and Renewable Source of Adult Stem Cells
15. A Simplified and Systematic Method to Isolate, Culture and Characterize Multiple Types of Human Dental Stem Cells from a Single Tooth
Mohammed Bakkar,  Younan Liu , Dongdong Fang, Camille Stegen,  Xinyun Su, Murali Ramamoorthi,  Li-Chieh Lin,  Takako Kawasaki , Nicholas Makhoul , Huan Pham , Yoshinori Sumita, and Simon D. Tran

16. Measurement of Autophagy by Flow Cytometry
Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia

17. Echocardiography-Guided Intramyocardial Injection Method in a Murine Model
Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen

18. Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells
Federico Franchi and Martin Rodriguez-Porcel

19. Methods for Long Term Storage of Murine Bone Marrow Derived Mesenchymal Stem Cells
Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra

20. Therapeutic Application of Adult Stem Cells in the Heart
Taylor A. Johnson and Dinender K. Singla

21. Patient-Derived and Intra-Operatively Formed Biomaterial for Tissue Engineering
Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu

22. Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering

  Dongdong Fang, Michael Roskies , Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran

23. Optimal Environmental Stiffness for Stem Cell Mediated Ischemic Myocardium Repair
Honghai Liu, Christian Paul, and Meifeng Xu

24. Assessment of MiRNA Regulation of Endothelial Progenitor Cell Mediated Angiogenesis
Darukeshwara Joladarashi and Prasanna Krishnamurthy

25. Stem Cell Exosomes: Cell Free-Therapy for Organ Repair
Mohsin Khan and Raj Kishore

Blurb

This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments.