Adult Stem Cells: Biology and Methods of Analysis

ISBN-13: 9781617790010 / Angielski / Twarda / 2011 / 279 str.

Donald G. Phinney

Opis

This is comprehensive overview of a vital area of scientific enquiry, which covers a broad spectrum of issues. With contributions from some of the key researchers in the field, Adult Stem Cells: Biology and Methods of Analysis offers readers a historical perspective as well as unique insights into cutting-edge thoughts. The volume contextualizes the recent discovery of stem/progenitor cell populations resident in many adult tissues and organs. It confronts the complexities scientists face in trying to validate these cells, while it also describes and critically evaluates the methods currently used to assess stem cell self-renewal. The chapters also seek to distinguish this process from other aspects of cell survival, such as the regulation of life span, senescence, and immortalization at a molecular level. The monograph begins with a section that examine the basic biology of adult stem cells, including chapters on the emerging role of microRNAs in regulating their fate and the molecular mechanisms that govern their self-renewal, the book moves on to analyze the varying methodologies employed in characterizing these elusive elements of our genetic make-up. The second section details in-vivo lineage tracing of tissue-specific stem cells, explores the neural stem cell paradigm, and considers the function of ABC transporters and aldehyde dehydrogenase in adult stem-cell biology. The final section shifts the focus to the life-span regulation and immortalization and features a chapter on the cancer stem cell paradigm.This is an authoritative volume on one of the frontiers of genetic research, and will serve as a valuable resource, not just for established scientists but also for those now entering the field of stem cell biology.

Recenzje

From the reviews:

"The three parts that makes up this volume, the first dealing with the basic biology, the second with the characterization of the adult stem cells phenotype and the third with the regulation of the life span of these cells, are all targeted to the critical evaluation of the techniques we use to distinguish adult stem cell-renewal from cell survival. ... The volume is well written and well illustrated ... . Well done!" (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Spis treści

 

Preface

Contributors

Corresponding Authors

Part I              Basic Biology of Adult Stem Cells               

Chapter 1       Molecular Mechanisms Regulating Adult Stem

                        Cell Self-Renewal

                        D. M. Panchision

 

Chapter 2       Maintenance of Adult Stem Cells: Role of the

Stem Cell Niche

                        T. Suda

Chapter 3       Emerging Role for microRNAs in Regulating

                        Adult Stem Cell Fate

J.M. Shookhoff and G.I. Gallicano

Chapter 4       Expression and Function of Pluripotency-Specific

                        Genes in Adult Stem Cells

                A. Lo Nigro, P. Roelandt and C. M. Verfaillie

 

Chapter 5       Adult Stem Cell Plasticity Revisited

                     E. Mezey

 

 

Part II             Characterization of Adult Stem Cells

Chapter 6       Lineage Tracing of Tissue-Specific Stem Cells

                        In Vivo

                        K. T. Sobush, K. Matsumoto, H. Chen, and

B. R.Stripp

 

Chapter 7       Surrogate Measure of Adult Stem Cells Self-

                        Rewal:  The Neural Stem Cell Paradigm

                L.P. Deleyrolle, B.A. Reynolds and

                        F.A. Siebzehnrubl

 

Chapter 8       ABC Transporters, Aldehyde Dehydrogenase,

                        And Adult Stem Cells

                        M. R. Allison

 

 

Part III           Regulation of Life Span and Immortalization

Chapter 9       Regulation of Life Span in Adult Stem Cells

                P. Davy and R. Allsopp

Chapter 10     The cancer stem cell paradigm

                    C. Eyler, J. Heddleston, S. Rhinos, M. Hitomi,

                        O. Guryanova, W. Flavahan, A. Hjelmeland, and

J.  Rich