Adult Heart Muscle Cells: Isolation, Properties and Applications

ISBN-13: 9783662110430 / Angielski / Miękka / 2013 / 166 str.

H. M. Piper; P. G. Spieckermann

Opis

With this volume results become generally available of a recent conference that was the first to discuss properties of isolated heart muscle cells from the adult myocardium as a new cardio- logical research tool. The conference was held as an International Erwin Riesch Symposium un- der the auspices of the Deutsche Gesellschaft fUr Herz- und Kreislaufforschung at Spangenberg Castle, March 22-25, 1984. It was made possible through funding by the Erwin Riesch Foun- dation and the Bayer AG which deserve our gratitude for their generosity. It may not be fully reflected in this collection of papers that the atmosphere of this meeting was unique. The reason for this resided apparently in the still rudimentary experiences with the adult cardiocyte model that is now widely acknowledged to be very promising. Thus, quite basic inter- ests brought together experts from different biological disciplines who in respect to this new mod- el were all still "beginners". It was noteworthy that such common interests exerted a unifying effect upon otherwise rather separated fields of science. The main task of the years to come will certainly consist in improving isolation and culturing conditions for adult myocytes in order to fully establish the isolated cell model in heart research.

Spis treści

Isolated adult heart cells. The development of a new experimental model.- Ca-tolerant guinea-pig ventricular myocytes as isolated by pronase in the presence of 250 ?M free calcium.- Guinea-pig atrial cardioballs.- Determination of isolated myocyte viability: Staining methods and functional criteria.- Sarcolemma-bound calcium. Its importance for cell viability.- Anoxic injury of adult cardiac myocytes.- Non-invasive measurements of cell surface receptors.- Surface antigens of adult heart cells and their use in diagnosis.- Substrate utilization of adult cardiac myocytes.- Characteristics of lactate transfer in isolated cardiac myocytes.- The insulin receptor of adult heart muscle cells.- Kinetics of cellular uptake of tracers used in myocardial scintigraphy.- Adhesion of cardiac myocytes to extracellular matrix components.- Development of new intercellular contacts between adult cardiac myocytes in culture.- Long-term primary cultures of adult human and rat cardiomyocytes.- Morphological dedifferentiation of adult cardiac myocytes in coculture with hepatocytes.- Electrophysiological properties of isolated ventricular myocytes.- The effect of cyanide on the K-current in guinea-pig ventricular myocytes.- Measurements of single-channel currents in the membrane of isolated cells: ATP-dependence of K+-channels.- Evidence for Ca-mediated inactivation of ICa in dialysed guinea-pig atrial cardioballs.- Cardiac membrane currents and energetic state.- Simultaneous measurements of action potentials and contractions in single cultured adult and embryonic heart muscle cells.- The two components in the shortening of unloaded ventricular myocytes: Their voltage dependence.- Temperature dependence of verapamil action.- Microtubules and desmin filaments during the onset of heart growth in the rat.- The correlation between catecholamine and lipid peroxidation induced damage in heart cells.- Anoxia in neonatal rat heart cell cultures.- Enzyme release and glycolytic energy production.- Anoxia influences the lateral diffusion of a lipid probe in the plasma membrane of isolated cardiac myocytes.- Summaries of the discussions of the symposium.- Abstracts of the poster-session on the adult myocyte model.- Author Index.