Adenovirus: Methods and Protocols

ISBN-13: 9781627036788 / Angielski / Twarda / 2013 / 247 str.

Miguel Chillon; Assumpcio Bosch

Opis

Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.

Spis treści

1. Biophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle

            Rosa Menéndez-Conejero, Ana J. Pérez-Berná, Gabriela N. Condezo, Alvaro Ortega-Esteban, Marta del Alamo, Pedro J. de Pablo, and Carmen San Martín

 

2. Proteome Analysis of Adenovirus Using Mass Spectrometry

            Sara Bergström Lind, Konstantin A. Artemenko, and Ulf Pettersson

 

3. Capsid Modification Strategies for Detargeting Adenoviral Vectors

            Alan L. Parker, Angela C. Bradshaw, Raul Alba, Stuart A. Nicklin, and Andrew H. Baker

 

4. Use of Dodecahedron “VLPs” as an Alternative to the Whole Adenovirus

            Pascal Fender

 

5. Study of Adenovirus and CAR Axonal Transport in Primary Neurons

            Charleine Zussy and Sara Salinas

 

6. The Use of Chromatin Immunoprecipitation (ChIP) to Study the Binding of Viral Proteins to the Adenovirus Genome In Vivo

            Yueting Zheng and Patrick Hearing

 

7. DNA Microarray to Analyze Adenovirus-Host Interactions

            Stefania Piersanti, Enrico Tagliafico, and Isabella Saggio

 

8. Determination of the Transforming Activities of Adenovirus Oncogenes

            Thomas Speiseder, Michael Nevels, and Thomas Dobner

 

9. Oncolytic Adenovirus Characterization: Activity and Immune Responses

            Raul Gil-Hoyos, Juan Miguel-Camacho, and Ramon Alemany

 

10. The Analysis of Innate Immune Response to Adenovirus Using Antibody Arrays

            Nelson C. Di Paolo and Dmitry M. Shayakhmetov

 

11. Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology

            Zsolt Ruzsics, Frederic Lemnitzer, and Christian Thirion

 

12. Construction, Production, and Purification of Recombinant Adenovirus Vectors

            Susana Miravet, Maria Ontiveros, Jose Piedra, Cristina Penalva, Mercè Monfar, and Miguel Chillón

 

13. Scalable Production of Adenovirus Vectors

            Ana Carina Silva, Paulo Fernandes, Marcos F.Q. Sousa, and Paula M. Alves

 

14. Canine Adenovirus Downstream Processing Protocol

            Meritxell Puig, Jose Piedra, Susana Miravet, and María Mercedes Segura

 

15. Production of High-Capacity Adenovirus Vectors

            Florian Kreppel

 

16. Production of Chimeric Adenovirus

            Marta Miralles, Marc Garcia, Marcos Tejero, Assumpció Bosch, and Miguel Chillón