Adeno-Associated Virus Vectors: Design and Delivery

ISBN-13: 9781493991389 / Angielski / Twarda / 2019 / 426 str.

Michael J. Castle

Spis treści

Part I: Gene Delivery to the Central Nervous System

 

Chapter 1. Stereotactic Intraparenchymal Injection for Gene Delivery to the Brain

 

Chapter 2. MRI-Guided Intraparenchymal Injection for Gene Delivery to the Brain (Krystof Bankiewicz)

 

Chapter 3: Intravenous Infusion for Widespread Delivery to Nervous and Peripheral Tissues (Brian Kaspar)

 

Chapter 4: MRI-Guided Focused Ultrasound for Targeted Gene Delivery to the Brain (Isabelle Aubert)

 

Chapter 5: Intrathecal Infusion for Gene Delivery to the Nervous System (Mark Tuszynski and myself)

           

Chapter 6: Direct Intraparenchymal Injection for Gene Delivery to the Spinal Cord

 

Chapter 7: Retrograde Targeting of Spinal Motor Neurons by Muscle Injection

 

Chapter 8: Subpial Infusion for Widespread Gene Delivery to the Spinal Cord

 

Chapter 9: Intraganglionic Microinjection for Gene Delivery to the DRG

 

Chapter 10: Intravitreal Injection for Gene Delivery to the Eye

 

Chapter 11: Subretinal Injection for Gene Delivery to the Eye (Luk Vandenberghe)

 

[Chapters 10 and 11 could be combined into a single chapter for ocular delivery]

 

Part II: Gene Delivery to Peripheral Tissues

 

Chapter 12: Intranasal Administration for Gene Delivery to the Lung

 

Chapter 13: Orotracheal Administration for Gene Delivery to the Lung

 

Chapter 14: Surgical Tracheal Administration for Gene Delivery to the Lung

 

[Chapters 13 and 14 could be combined into a single chapter for tracheal delivery]

Chapter 15: Infusion of Coronary Artery for Global Gene Delivery to the Heart

Chapter 16: Aortic Occlusion and Pressurized Infusion for Global Gene Delivery to the Heart

[Chapters 15 and 16 could be combined into a single chapter for global heart delivery]

 

Chapter 17: Intramyocardial Injection for Targeted Gene Delivery to the Heart

 

Chapter 18: Intraportal Vein Injection for Global Gene Delivery to the Liver

 

Chapter 19: Intraparenchymal Injection for Targeted Gene Delivery to the Liver

 

Chapter 20: Gene Delivery to the Spleen

 

Chapter 21: Gene Delivery to the Kidney

 

Chapter 22: Gene Delivery to Skeletal Muscle

 

Part III: Design of Novel Vectors for Targeting of Specific Tissues and Cell Types

 

Chapter 23: Design of AAV vectors for Delivery of Large or Multiple Transgenes

 

Chapter 24: Design of AAV vectors for Delivery of RNAi

 

Chapter 25: Altering AAV tropism with Directed Evolution

 

Chapter 26: Altering AAV tropism with AAV-CREATE (Ben Deverman)

 

Chapter 27: Altering AAV tropism with Phage Display (Tomas Bjorklund)

 

Chapter 28: Minipromoters for Tissue- and Cell-Specific Gene Expression (Elizabeth Simpson)

 

Blurb

This volume provides a complete and timely guide to the use of adeno-associated virus (AAV)  vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Adeno-Associated Virus Vectors: Design and Delivery aims to enhance the utility of AAV vectors for targeted gene transfer to living animals and continue the ongoing development of novel AAV-based gene therapies for human disease.