Adenine Nucleotides in Cellular Energy Transfer and Signal Transduction: UNESCO

ISBN-13: 9783034873178 / Angielski / Miękka / 2012 / 476 str.

Papa;Azzi;Tager

Opis

Adenine Nucleotides play a major role in cellular metabolism and functions, serving as high-potential phosphate transfer compounds in energy metabolism and as substrates and co-factors for proteins involved in signal transduction. During the last few years definite advancement has been made in elucidating the molecular and genetic aspects of the enzyme involved in oxidative phosphorylation, the so-called FoFl H -ATP synthase. Non-invasive NMR technologies have been developed to monitor in vivo the energy level of tissues based on determination of the concentrations of adenine nucleotides, phosphate and phosphate esters. Thus it became clear that the capacity of oxidative phosphorylation adapts itself to the ATP demand which changes continuously with the physiological state in various tissues. This is achieved by regulation of the enzyme activity as well as by regulation of its biogenesis. The reversible phosphorylation of proteins is re- cognised as a major regulatory mechanism in eukaryotic as well as in prokaryotic cells for cellular signal transduction and control of gene expression, cell growth, differentation and oncogenesis. The same applies to the role played by cAMP. A further topic of growing interest concerns the discovery of the ATP binding cassette (ABC) superfamily of transport proteins which includes systems of primary importance in medicine such as the multi-drug resistance P glycoprotein, the cystic fibrosis transmembrane conductance regulator (CFTR) and the 70 kd peroxisomal membrane protein. Finally, much attention is being devoted in many laboratories to the molecular structure and role of ATP- modulated channels.

Spis treści

Adenine Nucleotides in Energy Transfer.- Molecular biology and assembly of yeast mitochondrial ATP synthase.- The mitochondrial H+-ATP synthase: Polypeptide subunits, proton translocating and coupling function.- Studies on the structure of the mitochondrial ATP synthase complex and the mechanism of ATP synthesis.- Number, localisation and function of the non-catalytic adenine nucleotide binding sites of mitochondrial F1-ATPase.- Fluoroaluminium and fluoroberyllium complexes as probes of the catalytic sites of mitochondrial F1-ATPase.- Regulation of ATP synthesis in rat heart cells.- Uncoupling action of fatty acids on heart muscle mitochondria and submitochondrial particles is mediated by the ATP/ADP antiporter.- Mitochondrial function and biogenesis in cultured mammalian cells without functional respiratory chains.- Regulation by adenine nucleotides, cyclic nucleotides and protein synthesis of the maturation of rat liver mitochondrial functions.- Inhibition of the mitochondrial ATPase by polyborate anions designed for neutron capture therapy.- 31p MR spectroscopic studies of metabolic lesions in the liver.- Mitochondrial mutations and the ageing process.- Structural and functional alterations of mitochondrial F0F1 ATP synthase in various pathophysiological states.- Long range intramolecular linked functions in the Ca2+ transport ATPase of intracellular membranes.- Synthesis and characterisation of radiolabeled azido-derivatives of P1, P5-Di (Adenosine-5?) pentaphosphate for mapping studies of adenylate kinase.- Protein Kinases.- Protein phosphorylation and the regulation of sugar transport in gram-negative and gram-positive bacteria.- Signal transduction of the phosphate regulon in Escherichia coli mediated by phosphorylation.- The protein kinase C family in signal transduction and cellular regulation.- Structure-function studies on the protein kinase C family members.- Mechanism of protein kinase C-mediated signal transduction.- Structural studies on the protein kinase C gene family.- ?-tocopherol inhibits smooth muscle cell proliferation and protects against arteriosclerosis.- Protein kinase C and G proteins.- Casein kinases: An atypical class of ubiquitous and pleiotropic protein kinases.- Spectrophotometric assay of protein kinases.- KGF receptor: Transforming potential on fibroblasts and epithelial cell-specific expression by alternative splicing.- The receptor for the hepatocyte growth factor-scatter factor: Ligand-dependent and phosphorylation-dependent regulation of kinase activity.- The kit receptor for the stem cell factor: Genetic lessons in signal transduction.- Adenylate Cyclase.- Liver adenylyl cyclases: Structure and regulation by cAMP-dependent phosphorylation.- Structural and functional organization of the catalytic domain of a bacterial toxin: Bordetella pertussis adenylate cyclase.- Signal transduction as studied in living cells by 31P-NMR: Adenylate cyclase activation and a novel phosphoethanolamine synthesizing pathway are stimulated by MSH in melanoma cells.- Adenylate cyclase-haemolysin toxin of Bordetella pertussis: Regulation of expression and activity.- Are purine nucleoside triphosphate cyclases an example of convergent evolution?.- Sensory transduction in dictyostelium.- The ATP Binding Cassette (ABC) Superfamily of Transport Proteins.- Structure and function of P-glycoproteins.- Function and regulation of the cystic fibrosis transmembrane conductance regulator.- The 70kDa peroxisomal membrane protein.- Latency of peroxisomal enzymes in digitonin-permeabilized cells: The effect of ATP on peroxisome permeability.- Dual action of fluorescein derivatives on KATP channels.- Control of synthesis of uncoupling protein and ATPase in animal and human brown adipose tissue.- Detection of the uncoupling protein (a nucleotide modulated H+-translocator) and its mRNA in human diseases.- Acknowledgements.