Activation tagging in transgenic poplar : Activation tagging in aspen using an inducible two component Ac/Ds-enhancer element system

ISBN-13: 9783639246674 / Angielski / Miękka / 2010 / 120 str.

Fadia El Sherif; Hamdy Kamal; Matthias Fladung

Opis

We describe the establishment of an activation tagging system in poplar based upon the maize Ac/Ds transposable element system. Transgenic poplar plants were obtained carrying either one or two of the above mentioned constructs [ Following induction of the GIP promoter only a weak transposase signal could be detected in RT- PCR experiments. Mobility of the ATDs activation construct could not be confirmed in the GIP system. [ An Activation Tagging experiment was initiated using eight different double transgenic lines. A few putative variants could be obtained revealing deficiency in chlorophyll content. [ Single transgenic aspen lines carrying HSP- transposase have been produced. Following transformation with HSP-transposase a chimerical transgenic line was obtained revealing white and green plants. Following activation tagging experiment a HSP-transposase/ATDs-rolC double transgenic line, one somaclonal variation line was obtained revealing white plants.