Accelerated Breeding of Cereal Crops

ISBN-13: 9781071615287 / Angielski / Miękka / 2022 / 338 str.

Andriy Bilichak; John D. Laurie

Opis

This volume provides a comprehensive collection of methods for plant breeders and researchers working in functional genomics of cereal crops. Chapters detail advances in sequencing of cereal genomes, methods of traditional plant breeding, use of machine learning for genomic selection, random and targeted mutagenesis with CRISPR/Cas9, quantitative proteomics and phenotyping in cereals. Authoritative and cutting-edge, Accelerated Breeding of Cereal Crops aims to be of  interest to plant breeders, researchers, postdoctoral fellows, and students working in functional genomics for the development of the next generation of crop plants.

Spis treści

1. Recent Advances In Sequencing of Cereal Genomes

Sean Walkowiak, Curtis J. Pozniak, and Kirby T. Nilsen

 

2. Mapping Quantitative Trait Loci in Wheat: Historic Perspective, Tools, and Methods For Analysis

Raman Dhariwal and Harpinder Randhawa

 

3. Introduction to Marker-Assisted Selection in Wheat Breeding

Sudhakar Pandurangan, Clare Workman, Kirby Nilsen, and Santosh Kumar

 

4. Genome-wide Association Studies (GWAS) in Cereals

Braulio J. Soto-Cerda, Akshaya Vasudevan, André Laroche, and Raja Ragupathy

  5. ropGBM: An Ultra-Efficient Machine Learning Toolbox For Genomic Selection-Assisted Breeding In Crops

Yuetong Xu, John D. Laurie, and Xiangfeng Wang

 

6. Doubled Haploid Production through Microspore Culture

Theia Myers, Kyle Bodell, Fengying Jiang, and John D. Laurie

 

7. Chemical and Physical Mutagenesis Approaches for Identification of Herbicide and Drought Tolerance Traits in Wheat

Neha Vaid, Marcus Samuel, Sateesh Kagale, and Raju Soolanayakanahally

 

8. Using TILLING Lines for Functional Genomics In Wheat

Liang Chen and Yin-Gang Hu

 

9. A Transposon-Based Activation Tag System for Functional Genomics in Cereals: Detection Of Mping-Based Activation Tag Mobilization In Wheat

Angela N. Plemmons, Amanda R. Askins, Sarah M. Mendoza, and C. Nathan Hancock

 

10. A Modular Cloning Toolkit for Genome Editing in Cereals

Florian Hahn, Laura Sanjurjo Loures, and Vladimir Nekrasov

 

11. Genome Editing and Identification of Targeted Heritable Mutations in Wheat

Matthew J. Milner and Emma J. Wallington

 

12. A Genotyping Protocol To Identify CRISPR/Cas9-edited Events in Hexaploid Wheat

Johann Schernthaner, Margaret Balcerzak, Jhadeswar Murmu, and Rajagopal Subramaniam

 

13. qPCR Methods for the Quantification Of Transgene Insert Copy Number and zygosity using the Comparative Ct method in transgenic Sorghum bicolor L. Moench

Joel Hague, Kimberly Nelson, Alex Yonchak, and Albert P. Kausch

 

14. A Roadmap for Undertaking Quantitative Proteomics in Plants: New Opportunities for Cereal Crops

 

Ibrahim Khodabocus, Qiaomu Li, Devang Mehta, and R. Glen Uhrig

 

15. DNA Affinity Purification Sequencing (DAP-seq) for Mapping Genome-Wide Transcription Factor Binding Sites in Plants

Miaomiao Li and Shao-shan Carol Huang

 

16. Basics of Sensor-based Phenotyping in Wheat

Emily Thoday-Kennedy, Nathan Good, and Surya Kant