4. Genome-wide Association Studies (GWAS) in Cereals
Braulio J. Soto-Cerda, Akshaya Vasudevan, André Laroche, andRaja Ragupathy
5. ropGBM: An Ultra-Efficient Machine Learning Toolbox For Genomic Selection-Assisted Breeding In Crops
Yuetong Xu, John D. Laurie, and Xiangfeng Wang
6. Doubled Haploid Production through Microspore Culture
Theia Myers, Kyle Bodell, Fengying Jiang, and John D. Laurie
7. Chemical and Physical Mutagenesis Approaches for Identification of Herbicide and Drought Tolerance Traits in Wheat
Neha Vaid, Marcus Samuel, Sateesh Kagale, and Raju Soolanayakanahally
8. Using TILLING Lines for Functional Genomics In Wheat
Liang Chenand Yin-Gang Hu
9. A Transposon-Based Activation Tag System for Functional Genomics in Cereals: Detection Of Mping-Based Activation Tag Mobilization In Wheat
Angela N. Plemmons, Amanda R. Askins, Sarah M. Mendoza, and C. Nathan Hancock
10. A Modular Cloning Toolkit for Genome Editing in Cereals
Florian Hahn, Laura Sanjurjo Loures, and Vladimir Nekrasov
11. Genome Editing and Identification of Targeted Heritable Mutations in Wheat
Matthew J. Milner and Emma J. Wallington
12. A Genotyping Protocol To Identify CRISPR/Cas9-edited Events in Hexaploid Wheat
Johann Schernthaner, Margaret Balcerzak, Jhadeswar Murmu, and Rajagopal Subramaniam
13. qPCR Methods for the Quantification Of Transgene Insert Copy Number and zygosity using the Comparative Ct method in transgenic Sorghum bicolor L. Moench
Joel Hague, Kimberly Nelson, Alex Yonchak, and Albert P. Kausch
14. A Roadmap for Undertaking Quantitative Proteomics in Plants: New Opportunities for Cereal Crops
Ibrahim Khodabocus, Qiaomu Li, Devang Mehta, and R. Glen Uhrig
15. DNA Affinity Purification Sequencing (DAP-seq) for Mapping Genome-Wide Transcription Factor Binding Sites in Plants
Miaomiao Li and Shao-shan Carol Huang
16. Basics of Sensor-based Phenotyping in Wheat
Emily Thoday-Kennedy, Nathan Good, and Surya Kant
This volume provides a comprehensive collection of methods for plant breeders and researchers working in functional genomics of cereal crops. Chapters detail advances in sequencing of cereal genomes, methods of traditional plant breeding, use of machine learning for genomic selection, random and targeted mutagenesis with CRISPR/Cas9, quantitative proteomics and phenotyping in cereals.
Authoritative and cutting-edge, Accelerated Breeding of Cereal Crops aims to be of interest to plant breeders, researchers, postdoctoral fellows, and students working in functional genomics for the development of the next generation of crop plants.