A Practical Guide to Monoclonal Antibodies

ISBN-13: 9780471929055 / Angielski / Miękka / 1991 / 206 str.

J. Eryl Liddell;Liddell; Philip Cryer

Opis

Focusing on monoclonal antibodies, this text covers such topics as immunization, hybrid selection and cloning, tissue culture techniques and an overview of hybridoma technology. The appendices provide information on media and buffers, sources of equipment and reagents, and a supplier's list.

Spis treści

Overview of Hybridoma Technology.

Tissue Culture Techniques.

Immunization.

Screening Test Design.

Myeloma Cells, Lymphocytes, Fusion.

Hybrid Selection and Cloning.

Characterization, Purification and Labeling.

Human Hybridomas and Engineered Antibodies.

Appendices.

References.

Index.

Blurb

A Practical Guide to Monoclonal Antibodies J. Eryl Liddell and A. Cryer Department of Biochemistry, University of Wales College of Cardiff, UK

This book has evolved as a result of the success of the post–experience courses in monoclonal antibody technology run by the Department of Biochemistry, University of Wales College of Cardiff. The authors have designed it to provide all the information required by a competent scientist to produce monoclonal antibodies, from basic tissue culture techniques, through immunisation strategies and screening test design, to the production of hybridoma cell lines and basic antibody characterisation, purification and labelling. The protocols are presented in a clearly distinguishable format for use at the laboratory bench. The concluding chapter provides an overview of the current status of human hybridoma production and antibody engineering using techniques of molecular biology. This book is essential reading for graduates in the biological sciences and in medicine who are involved in the making and using of monoclonal antibodies in commercial, university and medical laboratories.